First report of Achlya bisexualis infection in captive-reared Endangered golden mahseer Tor putitora.

IF 1.1 4区 农林科学 Q3 FISHERIES Diseases of aquatic organisms Pub Date : 2023-03-02 DOI:10.3354/dao03720
Victoria Chanu Khangembam, Dimpal Thakuria, Vinita Pant, Ritesh Shantilal Tandel, Bipin K Vishwakarma, Nityanand Pandey, Amit Pande, Pramod Kumar Pandey
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Abstract

Achlya bisexualis is a notorious oomycete pathogen with the potential to cause emerging disease in fish farms. In this study, we report the first isolation of A. bisexualis from captive-reared golden mahseer Tor putitora, an Endangered fish species. The infected fish showed a cotton-like growth of mycelia at the site of infection. The mycelium when cultured on potato dextrose agar produced radially growing white hyphae. The hyphae were non-septate, and some of them carried matured zoosporangium with dense granular cytoplasmic contents. Spherical gemmae with stout stalks were also observed. All the isolates had 100% identity in internal transcribed spacer (ITS)-rDNA sequence and showed highest similarity to that of A. bisexualis. In molecular phylogeny, all the isolates formed a monophyletic group with A. bisexualis which was supported by a bootstrap value of 99%. Based on the molecular and morphological findings, all the isolates were confirmed as A. bisexualis. Further, the anti-oomycete effect of boric acid, a known antifungal agent, against the isolate was evaluated. The minimum inhibitory concentration and minimum fungicidal concentration were found to be 1.25 and >2.5 g l-1, respectively. Isolation of A. bisexualis from a new fish species indicates its possible occurrence in other unreported hosts. Considering its wide infectivity and the potential to cause disease in farmed fishes, its probable prevalence in a new environment and host needs to be closely monitored to prevent the spread of infection, if any, by adopting suitable control measures.

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人工饲养的濒危金麝猫感染双性Achlya的首例报道。
双性Achlya是一种臭名昭著的卵菌病原体,有可能在养鱼场引起新发疾病。在本研究中,我们报道了首次从人工饲养的濒危物种金马尾鱼中分离到双性棘虫。受感染的鱼在感染部位菌丝体呈棉花样生长。菌丝体在马铃薯葡萄糖琼脂上培养时产生径向生长的白色菌丝。菌丝不分离,部分菌丝携带成熟的游动孢子囊,胞质致密颗粒状。茎粗的球形芽也被观察到。所有分离株在ITS -rDNA序列上具有100%的同源性,与双性田鼠的相似性最高。在分子系统发育上,所有分离株与双性芽孢杆菌形成一个单系群,自举率为99%。经分子和形态学分析,所有分离株均为双性单胞杆菌。进一步,对硼酸(一种已知的抗真菌剂)对该分离物的抗卵霉菌作用进行了评价。最小抑菌浓度为1.25 g -1,最小杀菌浓度为>2.5 g -1。从一种新鱼类中分离出双性棘虫,提示其可能存在于其他未报道的寄主中。考虑到其广泛的传染性和在养殖鱼类中引起疾病的可能性,需要密切监测其在新环境和宿主中的可能流行情况,以采取适当的控制措施防止感染的传播。
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来源期刊
Diseases of aquatic organisms
Diseases of aquatic organisms 农林科学-兽医学
CiteScore
3.10
自引率
0.00%
发文量
53
审稿时长
8-16 weeks
期刊介绍: DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically: -Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens -Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)- Diseases due to internal circumstances (innate, idiopathic, genetic)- Diseases due to proliferative disorders (neoplasms)- Disease diagnosis, treatment and prevention- Molecular aspects of diseases- Nutritional disorders- Stress and physical injuries- Epidemiology/epizootiology- Parasitology- Toxicology- Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)- Diseases as indicators of humanity''s detrimental impact on nature- Genomics, proteomics and metabolomics of disease- Immunology and disease prevention- Animal welfare- Zoonosis
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