Exploring phytochemical constituents of Achillea arabica Kotschy. ethanolic flower extract by LC-MS/MS and its possible antioxidant and antidiabetic effects in diabetic rats.

IF 1.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences Pub Date : 2023-05-25 DOI:10.1515/znc-2022-0082

Hanife Ceren Hanalp, Abdulahad Dogan, Tuba Kusman Saygi, Fatih Donmez, Abdulhamit Battal

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引用次数: 2

Abstract

The aim of this study was to reveal the antidiabetic and antioxidant effects of ethanolic lyophilized extract of Achillea arabica flower extract against streptozotosine (STZ)-induced in diabetic rats and to determine its phytochemical content by liquid chromatography with tandem mass spectrometry (LC-MS/MS). After toxicity test, 35 female rats were divided into five groups. Control, diabetes mellitus (DM), A.arabica (400 mg/kg) extract, DM + A. arabica (400 mg/kg) extract and DM + Glibenclamide (2 mg/kg). It was determined that while diabetic rats treated A.arabica plant extract significantly decreased blood glucose level, serum glucose, HbA1c, liver and kidney damage biomarker levels, and malondialdehyde (MDA) content compared to the DM group, it caused fluctuations in antioxidant enzyme levels. According to LC-MS/MS results of A. arabica flower extract, quinic acid (2439.9 μg/g), cyranoside (858.4 μg/g), chlorogenic acid (698.7 μg/g), and cosmosiin (347.8 μg/g) were determined as major compounds, respectively. In addition, two new compounds were determined in this extract according to nuclear magnetic resonance (NMR) and Mass analyses and these compounds were named edremitine and achillosine, respectively. Thus, A.arabica flower extract has possible therapeutic effects to prevent high blood glucose level and oxidative stress caused by DM in liver and kidney via its high phenolic content.

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阿拉比卡阿喀琉叶植物化学成分的研究。用LC-MS/MS分析乙醇花提取物对糖尿病大鼠的抗氧化和降糖作用。

摘要本研究旨在探讨阿拉伯藜花提取物乙醇冻干提取物对糖尿病大鼠STZ诱导的抗糖尿病和抗氧化作用，并采用液相色谱-串联质谱法(LC-MS/MS)测定其植物化学成分含量。毒性试验后，将35只雌性大鼠分为5组。对照组，糖尿病(DM)，阿拉比卡咖啡(400 mg/kg)提取物，DM +阿拉比卡咖啡(400 mg/kg)提取物和DM +格列本脲(2 mg/kg)。结果表明，与DM组相比，阿拉比卡植物提取物可显著降低糖尿病大鼠的血糖水平、血清葡萄糖、HbA1c、肝肾损伤生物标志物水平和丙二醛(MDA)含量，但会引起抗氧化酶水平的波动。根据阿拉比卡花提取物的LC-MS/MS结果，主要化合物分别为奎宁酸(2439.9 μg/g)、昔兰诺苷(858.4 μg/g)、绿原酸(698.7 μg/g)和宇宙花素(347.8 μg/g)。此外，经核磁共振和质谱分析，在该提取物中鉴定出两个新化合物，分别命名为edremitine和achillosine。因此，阿拉比卡花提取物可能通过其高酚类物质含量来预防DM引起的高血糖和肝脏和肾脏氧化应激。

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来源期刊

Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences 生物-生化与分子生物学

CiteScore

4.10

自引率

5.00%

发文量

期刊介绍： A Journal of Biosciences: Zeitschrift für Naturforschung C (ZNC) is an international scientific journal and a community resource for the emerging field of natural and natural-like products. The journal publishes original research on the isolation (including structure elucidation), bio-chemical synthesis and bioactivities of natural products, their biochemistry, pharmacology, biotechnology, and their biological activity and innovative developed computational methods for predicting the structure and/or function of natural products. A Journal of Biosciences: Zeitschrift für Naturforschung C (ZNC) welcomes research papers in fields on the chemistry-biology boundary which address scientific ideas and approaches to generate and understand natural compounds on a molecular level and/or use them to stimulate and manipulate biological processes.