{"title":"Comparative transcriptome analysis of wild type and a pectate lyase mutant strain of Bacillus subtilis.","authors":"Kongyang Wu, Jinqiu Wu, Xue Li, Tongxiang Yang, Xuedong Chen, Xueyi Yang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Transcriptome analysis for the original Bacillus subtilis K1 strain and UV mutagenic strain UW07 with high yield of pectate lyase was implemented with RNA-seq. The function of genes was annotated and metabolic pathways were classified to look for different expression genes and classify these genes into related metabolic pathways to reveal the high-yield mechanism of pectate lyase in UW07. The results showed that 397 genes were up-regulated and 617 genes were down-regulated compared with the original strain. The up-regulated genes were mainly involved in ABC transporters, two-component system, biosynthesis of amino acids, and carbon metabolism.</p>","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"46 1","pages":"52-55"},"PeriodicalIF":1.5000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Microbiologica","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Transcriptome analysis for the original Bacillus subtilis K1 strain and UV mutagenic strain UW07 with high yield of pectate lyase was implemented with RNA-seq. The function of genes was annotated and metabolic pathways were classified to look for different expression genes and classify these genes into related metabolic pathways to reveal the high-yield mechanism of pectate lyase in UW07. The results showed that 397 genes were up-regulated and 617 genes were down-regulated compared with the original strain. The up-regulated genes were mainly involved in ABC transporters, two-component system, biosynthesis of amino acids, and carbon metabolism.
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The publication, diffusion and furtherance of research and study on all aspects of basic and clinical Microbiology and related fields are the chief aims of the journal.
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