Genetic Clarification of Auricularia heimuer Strains Bred and Cultivated in Korea Using the ITS and IGS1 rDNA Region Sequences.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.1080/12298093.2023.2182024

Nitesh Pant, HyeongJin Noh, Won-Ho Lee, Seong Hwan Kim

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Abstract

Auricularia is one of the broadly cultivated edible mushrooms in Korea. Most of the Korean Auricularia strains used for cultivation and breeding are known as A. auricula-judae. Recently, this species has been reported to belong to a species complex. Therefore, this study was carried out to genetically clarify the bred and cultivated Korean A. auricula-judae strains. The internal transcribed spacer (ITS) and IGS1 rDNA region sequences were determined from 10 A. auricula-judae strains by PCR and sequencing. Variation in the nucleotide sequence and sequence length of the two rDNA regions were found among the seven A. auricula-judae strains. A maximum-likelihood (ML) phylogenetic tree based on the ITS sequences clearly placed all the 10 Korean A. auricula-judae strains in the A. heimuer clade of the A. auricula-judae complex. A. heimuer is diverged from A. auricula-judae. An ML phylogenetic tree based on the IGS1 sequences revealed the close relationship between Korean A. heimuer strains to Chinese A. heimuer strains. But each strain could be distinguishable by the IGS1 sequence. Furthermore, progeny strains in the seven Korean strains could be differentiated from their parental strains by the IGS1 sequence based phylogenetic tree. Our results are expected to be used to complement the distinction of domestic Auricularia cultivars.

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利用ITS和IGS1 rDNA区域序列对韩国黑木耳选育品系的遗传澄清

黑木耳是韩国广泛种植的食用菌之一。大多数用于栽培和育种的韩国黑木耳菌株被称为A. auricula-judae。最近，有报道称该物种属于一个物种复合体。因此，本研究对韩国黑穗病菌的育种和栽培菌株进行了遗传澄清。采用PCR和测序方法，测定了10株耳木耳菌株的ITS和IGS1 rDNA区域序列。这两个rDNA区域的核苷酸序列和序列长度在7株耳鼠菌株中存在差异。基于ITS序列的最大似然(ML)系统发育树将所有10株韩国木耳-judae菌株明确定位于木耳-judae复合体的heimuer支。黑木耳是由黑木耳分化而来。基于IGS1序列的ML系统进化树显示，韩国与中国的海姆氏杆菌亲缘关系较近。但每个菌株都可以通过IGS1序列进行区分。此外，基于IGS1序列的系统发育树可以区分7株韩国菌株的后代菌株与亲本菌株。本研究结果可作为我国黑木耳品种鉴别的补充。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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