Expression and Correlation of MIF and ERK1/2 in Liver Cirrhosis and Hepatocellular Carcinoma Induced by Hepatitis B.

IF 1.8 4区医学 Q3 PHARMACOLOGY & PHARMACY Pharmacogenomics & Personalized Medicine Pub Date : 2023-01-01 DOI:10.2147/PGPM.S398976

Dong Jia, Bin Li, Jun-Ke Wang, Pan Wang, Chu-Yi Li, Li-Xia Lu, Wen-Yan Tian, Xiao-Hui Yu, Jiu-Cong Zhang, Ying Zheng

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Abstract

Objective: To detect expression and phosphorylation level of macrophage migration inhibitor (MIF) and extracellular-regulated kinases 1 and 2 (ERK1/2) in hepatitis B-induced liver cirrhosis (HBILC) and hepatocellular carcinoma (HCC) with a background of HBILC and analyze the correlation of MIF and ERK1/2 with HBILC and HCC.

Methods: Twenty cases of normal liver tissues were collected as a control group, and 48 specimens of HBILC tissues and 48 specimens of HCC tissues were collected as the experimental group, which were assigned as the HBILC group and HCC group, respectively. All tissue specimens were processed into tissue chips. The expressions of MIF, ERK1/2, and their phosphorylated proteins were detected via immunohistochemistry, and MIF and ERK1/2 nucleic acid expressions were detected by in situ hybridization. The results were statistically analyzed using the chi-square test.

Results: Proteins and nucleic acids of MIF and ERK1/2 presented low expression in the control group and high expression in the HBILC group and HCC group. MIF expression in the three groups was 25.0%, 75.0%, and 79.17%, respectively, while that of the nucleic acids was 25.0%, 70.83%, and 68.75%, respectively. Expression of ERK1/2 in the three groups was 40.0%, 60.42%, and 81.25%, respectively, and that of nucleic acids was 40.0%, 79.17%, and 77.08%. Expression of pERK1/2 was low in the control and HBILC group and high in the HCC group. Expression of pERK1/2 in the three groups was 20%, 45.83%, and 75%, respectively. Expression of pERK1/2 in the HCC group was significantly different from that in the HBILC and control group (P<0.05), but the difference between the HBILC group and control group was not statistically significant (P>0.05).

Conclusion: Occurrence and development of HBILC and HCC are not only related to the high expression of MIF but also closely related to the activation of the ERK1/2 signaling pathway.

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MIF和ERK1/2在乙型肝炎诱导的肝硬化和肝细胞癌中的表达及相关性

目的:检测巨噬细胞迁移抑制剂(MIF)和细胞外调节激酶1和2 (ERK1/2)在HBILC背景下乙型肝炎肝硬化(HBILC)和肝细胞癌(HCC)中的表达和磷酸化水平，分析MIF和ERK1/2与HBILC和HCC的相关性。方法:选取20例正常肝组织作为对照组，选取48例HBILC组织和48例HCC组织作为实验组，分别分为HBILC组和HCC组。所有组织标本均加工成组织芯片。免疫组化检测MIF、ERK1/2及其磷酸化蛋白表达，原位杂交检测MIF、ERK1/2核酸表达。结果采用卡方检验进行统计学分析。结果:MIF和ERK1/2蛋白及核酸在对照组呈低表达，在HBILC组和HCC组呈高表达。三组MIF表达量分别为25.0%、75.0%和79.17%，核酸表达量分别为25.0%、70.83%和68.75%。ERK1/2在三组中的表达量分别为40.0%、60.42%和81.25%，核酸的表达量分别为40.0%、79.17%和77.08%。pERK1/2在对照组和HBILC组低表达，在HCC组高表达。三组中pERK1/2的表达量分别为20%、45.83%和75%。pERK1/2在HCC组的表达与HBILC组及对照组比较差异有统计学意义(PP>0.05)。结论:HBILC和HCC的发生发展不仅与MIF的高表达有关，而且与ERK1/2信号通路的激活密切相关。

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来源期刊

Pharmacogenomics & Personalized Medicine Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

3.30

自引率

5.30%

发文量

110

审稿时长

16 weeks

期刊介绍： Pharmacogenomics and Personalized Medicine is an international, peer-reviewed, open-access journal characterizing the influence of genotype on pharmacology leading to the development of personalized treatment programs and individualized drug selection for improved safety, efficacy and sustainability. In particular, emphasis will be given to: Genomic and proteomic profiling Genetics and drug metabolism Targeted drug identification and discovery Optimizing drug selection & dosage based on patient''s genetic profile Drug related morbidity & mortality intervention Advanced disease screening and targeted therapeutic intervention Genetic based vaccine development Patient satisfaction and preference Health economic evaluations Practical and organizational issues in the development and implementation of personalized medicine programs.