Identification of Key Target Genes and Pathway Analysis in Nonalcoholic Fatty Liver Disease Via Integrated Bioinformatics Analysis.

IF 0.5 4区医学 Q4 GENETICS & HEREDITY Balkan Journal of Medical Genetics Pub Date : 2022-06-01 DOI:10.2478/bjmg-2022-0006

X Chen, L Zhang, Y Wang, R Li, M Yang, L Gao

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引用次数: 1

Abstract

Purpose: This study aimed at exploring the mechanisms underlying nonalcoholic fatty liver disease (NAFLD) and developing new diagnostic biomarkers for nonalcoholic steatohepatitis (NASH).

Methods: The microarray dataset GES83452 was downloaded from the NCBI-GEO database, and the differentially expressed RNAs (DERs) were screened between the NAFLD and non-NAFLD samples of the baseline and 1-year follow-up time point group based on the Limma package.

Results: A total of 561 DERs (268 downregulated and 293 upregulated) were screened in the baseline time point group, and 1163 DERs (522 downregulated and 641 upregulated) were screened in the 1-year follow-up time point group. A total of 74 lncRNA-miRNA pairs and 523 miRNA-mRNA pairs were obtained in order to construct a lncRNA-miRNA-mRNA regulatory network. Subsequently, functional enrichment analysis revealed 28 GO and 9 KEGG pathways in the ceRNA regulatory network. LEPR and CXCL10 are involved in the Cytokine-cytokine receptor interaction (P = 1.86E-02), and the FOXO1 is involved in both the insulin signaling pathway (P = 1.79E-02) and the pathways in cancer (P = 2.87E-02).

Conclusion: LEPR, CXCL10, and FOXO1 were the characteristic target genes for NAFLD.

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基于综合生物信息学分析的非酒精性脂肪肝关键靶基因鉴定及通路分析

目的:本研究旨在探讨非酒精性脂肪性肝病(NAFLD)的发病机制，并开发新的非酒精性脂肪性肝炎(NASH)的诊断生物标志物。方法:从NCBI-GEO数据库下载微阵列数据集GES83452，基于Limma包筛选基线组和随访1年时间点组NAFLD和非NAFLD样本之间的差异表达rna (DERs)。结果:基线时间点组共筛选到561例der(下调268例，上调293例)，随访1年时间点组共筛选到1163例der(下调522例，上调641例)。为了构建lncRNA-miRNA- mrna调控网络，共获得74对lncRNA-miRNA和523对miRNA-mRNA。随后，功能富集分析揭示了ceRNA调控网络中的28个GO通路和9个KEGG通路。LEPR和CXCL10参与细胞因子-细胞因子受体相互作用(P = 1.86E-02)， FOXO1参与胰岛素信号通路(P = 1.79E-02)和癌症通路(P = 2.87E-02)。结论:LEPR、CXCL10和FOXO1是NAFLD的特征性靶基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Balkan Journal of Medical Genetics 医学-遗传学

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Balkan Journal of Medical Genetics is a journal in the English language for publication of articles involving all branches of medical genetics: human cytogenetics, molecular genetics, clinical genetics, immunogenetics, oncogenetics, pharmacogenetics, population genetics, genetic screening and diagnosis of monogenic and polygenic diseases, prenatal and preimplantation genetic diagnosis, genetic counselling, advances in treatment and prevention.