Granulocyte-colony stimulating factor mediates neovascularization in acellular dermal matrix-transplanted areas by promoting endothelial progenitor cell homing.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-05-01 DOI:10.4149/gpb_2023002

Jun Wang, Xuan Liao, Xiao Jiang, Hongwei Liu

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Abstract

Acellular dermal matrix (ADM) is an ideal material for tissue engineering skin construction. Accelerating the vascularization of ADM is of great significance for improving the survival of skin transplantation. The purpose of this study is to investigate the function of granulocyte-colony stimulating factor (G-CSF) in endothelial progenitor cells (EPCs)-mediated neovascularization in ADM-transplanted skin area. Male Kunming mice were subcutaneous injected with 10 μg/kg GCSF at 5 days before skin in situ replantation or porcine ADM transplantation. The surrounding tissues of implanted skin or venous blood was collected from the mice before the operation, and after the operation for 48 h, 72 h, 1 week, and 2 weeks, respectively. Cells co-expressing EPC markers, CD133, CD34, and Flk-1 were detected by flow cytometry. Immunohistochemistry of BrdU was performed to evaluate neovascularization in ADM-transplanted skin area. The results showed that G-CSF treatment increased the number of CD133+-CD34+ cells and CD133+-Flk-1+ cells in ADMimplanted area as well as the number of CD34+-Flk-1+ cells in peripheral blood. Likewise, G-CSF also increased the number of capillaries in ADM-transplanted areas. To sum up, G-CSF mobilizes EPC migration from bone marrow to peripheral blood and homing to wound sites, thus inducing neovascularization in ADM-transplanted areas.

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粒细胞集落刺激因子通过促进内皮祖细胞归巢介导脱细胞真皮基质移植区新生血管的形成。

脱细胞真皮基质(ADM)是组织工程皮肤构建的理想材料。加速ADM血管化对提高皮肤移植成活率具有重要意义。本研究旨在探讨粒细胞集落刺激因子(G-CSF)在adm移植皮肤内皮祖细胞(EPCs)介导的新生血管形成中的作用。雄性昆明小鼠于皮肤原位再植或猪ADM移植前5天皮下注射10 μg/kg GCSF。分别于术前、术后48 h、72 h、1周、2周采集小鼠植皮周围组织或静脉血。流式细胞术检测共表达EPC标记物、CD133、CD34和Flk-1的细胞。采用BrdU免疫组化方法评价adm移植皮肤区新生血管的形成情况。结果表明，G-CSF处理增加了adm植入区CD133+-CD34+细胞和CD133+-Flk-1+细胞的数量以及外周血CD34+-Flk-1+细胞的数量。同样，G-CSF也增加了adm移植区毛细血管的数量。综上所述，G-CSF动员EPC从骨髓迁移到外周血并归巢到伤口部位，从而诱导adm移植区域的新生血管形成。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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