Cholecalciferol affects cardiac proteins regulating malonyl-CoA availability and intracellular calcium level.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-05-01 DOI:10.4149/gpb_2023005

Tamara Ivkovic, Snezana Tepavcevic, Snjezana Romic, Mojca Stojiljkovic, Milan Kostic, Jelena Stanisic, Goran Koricanac, Tijana Culafic

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Abstract

Cholecalciferol improves insulin signaling and glucose metabolism in the heart and reduces circulating non-esterified fatty acids. Cholecalciferol effects on the cardiac fatty acid (FA) metabolism and the consequences on calcium handling were examined. Blood lipid profile was determined. Western blot and qRT-PCR were used to examine protein and mRNA expression. Cholecalciferoltreated rats had increased acetyl CoA carboxylase 2 protein expression and decreased expression of malonyl CoA decarboxylase. In addition, the expression of uncoupling protein 3 was elevated. Also, the level of peroxisome proliferator-activated receptor-gamma coactivator in the nucleus of heart cells was increased along with the level of sarcoplasmic/endoplasmic reticulum Ca2+ATPase in the microsomal fraction. In parallel, the L-type calcium channel and ryanodine receptor expression was reduced. In the heart of healthy rats, cholecalciferol affects proteins regulating malonyl CoA availability and intracellular Ca2+ handling proteins.

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胆钙化醇影响调节丙二酰辅酶a可用性和细胞内钙水平的心脏蛋白。

胆骨化醇改善胰岛素信号和心脏中的葡萄糖代谢，减少循环中的非酯化脂肪酸。研究了胆钙化醇对心脏脂肪酸(FA)代谢的影响以及对钙处理的影响。测定血脂水平。Western blot和qRT-PCR检测蛋白和mRNA的表达。胆钙化素处理大鼠乙酰辅酶a羧化酶2蛋白表达升高，丙二醛辅酶a脱羧酶表达降低。解偶联蛋白3的表达升高。此外，心脏细胞核中过氧化物酶体增殖体激活受体- γ辅激活因子的水平随着微粒体部分肌浆/内质网Ca2+ atp酶的水平而增加。同时，l型钙通道和ryanodine受体表达减少。在健康大鼠的心脏中，胆骨化醇影响调节丙二醇辅酶a可用性和细胞内Ca2+处理蛋白的蛋白质。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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