Investigation of the histopathological level of Ki-67, caspase-3 expressions of the effects of hesperidin on wound healing in the rat esophagus.

IF 1.1 4区医学 Q3 SURGERY Acta cirurgica brasileira Pub Date : 2023-01-01 DOI:10.1590/acb381723

Cemalettin Durgun, Gülsen Kirman, Engin Deveci

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Abstract

Purpose: The effects of hesperidin application on the wound caused by esophageal burns were investigated in this study.

Methods: Wistar albino rats were divided into three groups: Control group: only 1 mL of 0.09% NaCl was administered i.p. for 28 days; Burn group: An alkaline esophageal burn model was created with 0.2 mL of 25% NaOH orally by gavage-1 mL of 0.09% NaCl was administered i.p. for 28 days; Burn+Hesperidin group: 1 mL of 50 mL/kg of hesperidin was given i.p. for 28 days to rats after burn injury. Blood samples were collected for biochemical analysis. Esophagus samples were processed for histochemical staining and immunohistochemistry.

Results: Malondialdehyde (MDA) and myeloperoxidase (MPO) levels were significantly increased in Burn group. Glutathione (GSH) content and histological scores of epithelialization, collagen formation, neovascularization was decreased. After hesperidin treatment, these values were significantly improved in the Burn+Hesperidin group. In the Burn group, epithelial cells and muscular layers were degenerated. Hesperidin treatment restored these pathologies in Burn+Hesperidin group. Ki-67 and caspase-3 expressions were mainly negative in control group; however, the expression was increased in the Burn group. In the Burn+Hesperidin group, Ki-67 and caspase-3 immune activities were reduced.

Conclusions: Hesperidin dosage and application methods can be developed as an alternative treatment for burn healing and treatment.

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Ki-67、caspase-3表达水平对橙皮苷对大鼠食管创面愈合影响的研究。

目的:观察橙皮苷在食管烧伤创面的应用效果。方法:将Wistar白化大鼠分为3组:对照组:仅给予0.09% NaCl 1 mL, ig 28 d;烧伤组:0.25 % NaOH灌胃0.2 mL, 0.09% NaCl灌胃1 mL，造碱性食管烧伤模型28 d;烧伤+橙皮苷组:烧伤后大鼠ig橙皮苷50 mL/kg 1 mL，连续28 d。采集血样进行生化分析。对食管标本进行组织化学染色和免疫组织化学处理。结果:烧伤组大鼠丙二醛(MDA)、髓过氧化物酶(MPO)水平显著升高。谷胱甘肽(GSH)含量和上皮化、胶原形成、新生血管形成的组织学评分均降低。经橙皮苷治疗后，Burn+橙皮苷组上述指标均有显著改善。烧伤组上皮细胞和肌肉层变性。烧伤+橙皮苷组经橙皮苷治疗可恢复上述病理。对照组Ki-67、caspase-3表达以阴性为主;而Burn组表达增加。Burn+橙皮苷组大鼠Ki-67和caspase-3免疫活性降低。结论:橙皮苷的剂量和应用方法可作为烧伤愈合和治疗的替代治疗方法。

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