Effect of Hypoxia-Ischemia on the Expression of Iron-Related Proteins in Neonatal Rat Brains.

IF 3 4区 医学 Q2 NEUROSCIENCES Neural Plasticity Pub Date : 2023-01-01 DOI:10.1155/2023/4226139
Qing Lin, Ding-Wang Hu, Xin-Hui Hao, Geng Zhang, Ling Lin
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引用次数: 2

Abstract

Hypoxic-ischemic white matter injury (WMI) pathogenesis in preterm infants is not well established, and iron-related proteins in the brain may play an important role in imbalanced iron metabolism. We aimed to investigate the iron-related protein changes in neonatal rats after hypoxia-ischemia (HI), clarify the role of iron-related proteins in hypoxic-ischemic WMI, and potentially provide a new target for the clinical treatment of hypoxic-ischemic WMI in preterm infants. We adopted a WMI animal model of bilateral common carotid artery electrocoagulation combined with hypoxia in neonatal 3-day-old Sprague-Dawley rats. We observed basic myelin protein (MBP) and iron-related protein expression in the brain (ferritin, transferrin receptor [TfR], and membrane iron transporter 1 [FPN1]) via Western blot and double immunofluorescence staining. The expression of MBP in the WMI group was significantly downregulated on postoperative days (PODs) 14, 28, and 56. Ferritin levels were significantly increased on PODs 3, 7, 14, and 28 and were most significant on POD 28, returning to the sham group level on POD 56. FPN1 levels were significantly increased on PODs 7, 28, and 56 and were still higher than those in the sham group on POD 56. TfR expression was significantly upregulated on PODs 1, 7, and 28 and returned to the sham group level on POD 56. Immunofluorescence staining showed that ferritin, TfR, and FPN1 were expressed in neurons, blood vessels, and oligodendrocytes in the cortex and corpus callosum on POD 28. Compared with the sham group, the immune-positive markers of three proteins in the WMI group were significantly increased. The expression of iron-related proteins in the brain (ferritin, FPN1, and TfR) showed spatiotemporal dynamic changes and may play an important role in hypoxic-ischemic WMI.

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缺氧缺血对新生大鼠脑铁相关蛋白表达的影响。
早产儿缺氧缺血性脑白质损伤(WMI)的发病机制尚不明确,脑内铁相关蛋白可能在铁代谢失衡中起重要作用。我们旨在研究新生大鼠缺氧缺血(HI)后铁相关蛋白的变化,阐明铁相关蛋白在缺氧缺血性WMI中的作用,为临床治疗早产儿缺氧缺血性WMI提供新的靶点。采用3日龄新生大鼠双侧颈总动脉电凝联合缺氧的WMI动物模型。我们通过Western blot和双免疫荧光染色观察脑内碱性髓鞘蛋白(MBP)和铁相关蛋白(铁蛋白、转铁蛋白受体[TfR]和膜铁转运蛋白1 [FPN1])的表达。WMI组MBP的表达在术后14、28、56天显著下调。铁蛋白水平在POD 3、7、14和28上显著升高,在POD 28上最为显著,在POD 56上恢复到假手术组水平。FPN1水平在POD 7、28和56组显著升高,且仍高于POD 56组。在POD 1、7和28上,TfR表达显著上调,在POD 56上恢复到假手术组水平。免疫荧光染色显示,POD 28在皮层和胼胝体的神经元、血管和少突胶质细胞中表达铁蛋白、TfR和FPN1。与假手术组比较,WMI组三种蛋白免疫阳性标志物均显著升高。脑内铁相关蛋白(铁蛋白、FPN1和TfR)的表达呈现时空动态变化,可能在缺氧缺血性脑损伤中发挥重要作用。
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来源期刊
Neural Plasticity
Neural Plasticity NEUROSCIENCES-
CiteScore
6.80
自引率
0.00%
发文量
77
审稿时长
16 weeks
期刊介绍: Neural Plasticity is an international, interdisciplinary journal dedicated to the publication of articles related to all aspects of neural plasticity, with special emphasis on its functional significance as reflected in behavior and in psychopathology. Neural Plasticity publishes research and review articles from the entire range of relevant disciplines, including basic neuroscience, behavioral neuroscience, cognitive neuroscience, biological psychology, and biological psychiatry.
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