Molecular epidemiology, virulence and antimicrobial resistance of Bulgarian methicillin resistant Staphylococcus aureus isolates.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2022-09-16 DOI:10.1556/030.2022.01766

Raina Gergova, Virna-Maria Tsitou, Svetoslav G Dimov, Lyudmila Boyanova, Kalina Mihova, Tanya Strateva, Ivanka Gergova, Rumyana Markovska

{"title":"Molecular epidemiology, virulence and antimicrobial resistance of Bulgarian methicillin resistant Staphylococcus aureus isolates.","authors":"Raina Gergova, Virna-Maria Tsitou, Svetoslav G Dimov, Lyudmila Boyanova, Kalina Mihova, Tanya Strateva, Ivanka Gergova, Rumyana Markovska","doi":"10.1556/030.2022.01766","DOIUrl":null,"url":null,"abstract":"Background: Severe infections of virulent methicillin-resistant Staphylococcus aureus (MRSA) are a serious health problem. The present study aimed to investigate clonal spread, virulence and antimicrobial resistance rates of Bulgarian MRSA isolates in 2016-2020.Methods: Molecular identification and mecA gene detection were performed with PCR. Clonal relatedness was evaluated by RAPD PCR and MLST. MRSA epidemiology, virulence and resistance patterns were investigated by PCR.Results: All 27 isolates were identified as S. aureus and were mecA positive, and all were susceptible to linezolid, tigecycline and vancomycin. The toxin genes hlg (in 92.6% of isolates), seb (77.8%), sei (77.8%), seh (59.3%), sej (55.6%), and seg (48.1%), were frequently found among the isolates. Epidemiological typing by RAPD identified 4 clones (16 isolates) and 11 were with a unique profile. MLST analysis of the same MRSA isolates showed five MLST clonal complexes and 11 ST types, including CC5 (33.3%) (ST5, ST221, ST4776), CC8 (22.2%) (ST8, ST239, ST72), CC15 (ST582), CC22 (14.8%) (ST217, ST5417), CC30 (ST30) CC398 (ST398), and CC59 (ST59). The isolates from CC5 showed higher virulence potential and almost all were macrolide resistant (ermB or ermC positive). CC8 isolates showed higher level of resistance.Conclusion: To the best of our knowledge, this study is the first describing the clonal spreading of Bulgarian MRSA and the association with their virulence and resistance determinants. Monitoring of MRSA epidemiology, resistance and virulence profile can lead to better prevention and faster therapeutic choice in cases of severe infections.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1556/030.2022.01766","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 2

Abstract

Background: Severe infections of virulent methicillin-resistant Staphylococcus aureus (MRSA) are a serious health problem. The present study aimed to investigate clonal spread, virulence and antimicrobial resistance rates of Bulgarian MRSA isolates in 2016-2020.

Methods: Molecular identification and mecA gene detection were performed with PCR. Clonal relatedness was evaluated by RAPD PCR and MLST. MRSA epidemiology, virulence and resistance patterns were investigated by PCR.

Results: All 27 isolates were identified as S. aureus and were mecA positive, and all were susceptible to linezolid, tigecycline and vancomycin. The toxin genes hlg (in 92.6% of isolates), seb (77.8%), sei (77.8%), seh (59.3%), sej (55.6%), and seg (48.1%), were frequently found among the isolates. Epidemiological typing by RAPD identified 4 clones (16 isolates) and 11 were with a unique profile. MLST analysis of the same MRSA isolates showed five MLST clonal complexes and 11 ST types, including CC5 (33.3%) (ST5, ST221, ST4776), CC8 (22.2%) (ST8, ST239, ST72), CC15 (ST582), CC22 (14.8%) (ST217, ST5417), CC30 (ST30) CC398 (ST398), and CC59 (ST59). The isolates from CC5 showed higher virulence potential and almost all were macrolide resistant (ermB or ermC positive). CC8 isolates showed higher level of resistance.

Conclusion: To the best of our knowledge, this study is the first describing the clonal spreading of Bulgarian MRSA and the association with their virulence and resistance determinants. Monitoring of MRSA epidemiology, resistance and virulence profile can lead to better prevention and faster therapeutic choice in cases of severe infections.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

保加利亚耐甲氧西林金黄色葡萄球菌分离株的分子流行病学、毒力和抗菌素耐药性。

背景:恶性耐甲氧西林金黄色葡萄球菌(MRSA)的严重感染是一个严重的健康问题。本研究旨在调查2016-2020年保加利亚MRSA分离株的克隆传播、毒力和耐药性。方法:采用PCR方法进行分子鉴定和mecA基因检测。克隆亲缘性采用RAPD PCR和MLST分析。采用PCR方法研究MRSA流行病学、毒力及耐药模式。结果:27株分离株均为金黄色葡萄球菌，mecA阳性，对利奈唑胺、替加环素、万古霉素均敏感。毒素基因主要为hlg(92.6%)、seb(77.8%)、sei(77.8%)、seh(59.3%)、sej(55.6%)和seg(48.1%)。RAPD流行病学分型鉴定出4个克隆(16株)，其中11株具有独特的表型。对同一MRSA分离株进行MLST分析，发现5个MLST克隆复合物和11种ST类型，包括CC5 (33.3%) (ST5、ST221、ST4776)、CC8 (22.2%) (ST8、ST239、ST72)、CC15 (ST582)、CC22 (14.8%) (ST217、ST5417)、CC30 (ST30)、CC398 (ST398)和CC59 (ST59)。CC5菌株显示出较高的毒力潜力，几乎所有菌株都具有大环内酯耐药(ermB或ermC阳性)。CC8分离株表现出较高的耐药性。结论:据我们所知，这项研究首次描述了保加利亚MRSA的克隆传播及其与毒力和耐药性决定因素的关系。监测耐甲氧西林金黄色葡萄球菌流行病学、耐药性和毒力谱可在严重感染病例中更好地预防和更快地选择治疗方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.