Cellulosic Ethanol Production by Recombinant Cellulolytic Bacteria Harbouring pdc and adh II Genes of Zymomonas mobilis.

Biotechnology Research International Pub Date : 2012-01-01 DOI:10.1155/2012/817549

P Sobana Piriya, P Thirumalai Vasan, V S Padma, U Vidhyadevi, K Archana, S John Vennison

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引用次数: 17

Abstract

The ethanol fermenting genes such as pyruvate decarboxylase (pdc) and alcohol dehydrogenase II (adh II) were cloned from Zymomonas mobilis and transformed into three different cellulolytic bacteria, namely Enterobacter cloacae JV, Proteus mirabilis JV and Erwinia chrysanthemi and their cellulosic ethanol production capability was studied. Recombinant E. cloacae JV was found to produce 4.5% and 3.5% (v/v) ethanol, respectively, when CMC and 4% NaOH pretreated bagasse were used as substrates, whereas recombinant P. mirabilis and E. chrysanthemi with the same substrates could only produce 4%, 3.5%, 1%, and 1.5 % of ethanol, respectively. The recombinant E. cloacae strain produced twofold higher percentage of ethanol than the wild type. The recombinant E. cloacae strain could be improved further by increasing its ethanol tolerance capability through media optimization and also by combining multigene cellulase expression for enhancing ethanol production from various types of lignocellulosic biomass so that it can be used for industrial level ethanol production.

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含有活动单胞菌pdc和adh II基因的重组纤维素分解菌生产纤维素乙醇。

从活动单胞菌中克隆丙酮酸脱羧酶(pdc)和乙醇脱氢酶II (adh II)等乙醇发酵基因，并将其转化为阴沟肠杆菌、神奇变形杆菌和菊花Erwinia 3种不同的纤维素水解菌，研究了它们生产纤维素乙醇的能力。以CMC和4% NaOH预处理的甘蔗渣为底物时，重组阴沟芽孢杆菌的乙醇产量分别为4.5%和3.5% (v/v)，而以相同底物为底物的重组mirabilis和E.菊花的乙醇产量分别为4%、3.5%、1%和1.5%。重组阴沟肠杆菌菌株产生的乙醇百分比比野生型高两倍。重组阴沟芽孢杆菌菌株可以通过培养基优化提高其乙醇耐受性，并结合多基因纤维素酶表达提高不同类型木质纤维素生物质的乙醇产量，从而进一步提高其工业水平的乙醇生产能力。

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Biotechnology Research International

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