Characterization of 21 microsatellite loci for the precocious, grass-shrimp trematode Alloglossidium renale

IF 1.4 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular and biochemical parasitology Pub Date : 2023-06-01 DOI:10.1016/j.molbiopara.2023.111563

Jenna M. Hulke, Charles D. Criscione

引用次数: 0

Abstract

We developed microsatellite markers to use in studying the population genetics of the trematode Alloglossidium renale, a fluke with a precocious life cycle where sexual maturation occurs in a grass shrimp. Among 21 tested loci in a Mississippi population sample, 14 were polymorphic, 12 of which significantly deviated from Hardy-Weinberg Equilibrium (HWE). We estimated identity disequilibrium (ID) to confirm whether the deviations from HWE were due to significant amounts of selfing or due to technical factors. The selfing rate derived from F_IS was 86.6%, whereas the selfing rate obtained by ID was 83.9%, indicating that the deviation in HWE was due to a high amount of selfing within the population. These markers will be useful for ecological and evolutionary studies of A. renale especially in relation to the interplay of hermaphroditic mating systems, inbreeding depression, and transmission dynamics.

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早熟草虾异舌兰吸虫21个微卫星位点的鉴定

我们开发了微卫星标记，用于研究异舌兰吸虫的群体遗传学，异舌兰是一种具有早熟生命周期的吸虫，在草虾中发生性成熟。在密西西比州人群样本中的21个测试位点中，有14个是多态性的，其中12个显著偏离了Hardy-Weinberg平衡（HWE）。我们估计了身份不平衡（ID），以确认与HWE的偏差是由于大量的自拍还是由于技术因素。FIS得出的自拍率为86.6%，而ID获得的自拍率则为83.9%，这表明HWE的偏差是由于种群内的大量自拍造成的。这些标记物将有助于对A.renale的生态和进化研究，特别是与两性交配系统、近亲繁殖抑制和传播动力学的相互作用有关的研究。

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来源期刊

Molecular and biochemical parasitology 医学-寄生虫学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

63 days

期刊介绍： The journal provides a medium for rapid publication of investigations of the molecular biology and biochemistry of parasitic protozoa and helminths and their interactions with both the definitive and intermediate host. The main subject areas covered are: • the structure, biosynthesis, degradation, properties and function of DNA, RNA, proteins, lipids, carbohydrates and small molecular-weight substances • intermediary metabolism and bioenergetics • drug target characterization and the mode of action of antiparasitic drugs • molecular and biochemical aspects of membrane structure and function • host-parasite relationships that focus on the parasite, particularly as related to specific parasite molecules. • analysis of genes and genome structure, function and expression • analysis of variation in parasite populations relevant to genetic exchange, pathogenesis, drug and vaccine target characterization, and drug resistance. • parasite protein trafficking, organelle biogenesis, and cellular structure especially with reference to the roles of specific molecules • parasite programmed cell death, development, and cell division at the molecular level.