Cytogenetic and Molecular Characterization of Eigenmannia aff. desantanai (Gymnotiformes: Sternopygidae): A First Report of System of Sex Chromosomes ZW₁W₂/ZZ in Gymnotiformes.

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY Zebrafish Pub Date : 2023-04-01 DOI:10.1089/zeb.2022.0059

Laís de Araújo, Laura Ivana Ramos, Margarida Maria de Rossi Vieira, Alessandra Valéria de Oliveira, Ana Luiza de Brito Portela-Castro, Luciana Andreia Borin-Carvalho, Carlos Alexandre Fernandes

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引用次数: 1

Abstract

Gymnotiformes a monophyletic group of fish endemic to the Neotropics, represent an important component of the freshwater ichthyofauna that presents relevant taxonomic problems. Thus, in view of the morphological complexity involving Eigenmannia (Gymnotiformes) fish species, this study aimed to characterize Eigenmannia aff. desantanai of the upper Paraguay River basin through cytogenetic and molecular analyses, to help in the correct identification and delimitation of species. This study reports a multiple sex system of the type ZW₁W₂/ZZ, with 2n = 31 for females and 2n = 30 for males. A single pair of chromosomes carrying the nucleolar organizing regions (NORs) was detected. The heterochromatin was colocated in NOR sites and mainly located in the centromeric regions of chromosomes. Besides that, individual sequences COI from the specimens of E. aff. desantanai were obtained, totalizing three haplotypes. The distance p between the haplotypes in E. aff. desantanai, ranged from 0.2% to 7.1%. Species delimitation tests indicated the existence of two possible operational taxonomic units of E. aff. desantanai. Thus, this study reports a new multiple sex system in Gymnotiformes and these specimens previously identified as E. aff. desantanai may belong to two distinct species.

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裸子形目:胸骨蝶科本征甘菊的细胞遗传学和分子生物学特征:裸子形目性染色体ZW1W2/ZZ系统的首次报道。

裸子形目是新热带特有的单系鱼类，是淡水鱼系的重要组成部分，提出了相关的分类学问题。因此，鉴于本研究涉及的特征mannia(裸子形目)鱼类的形态复杂性，本研究旨在通过细胞遗传学和分子分析对巴拉圭河上游流域的特征mannia affr . desantanai进行表征，以帮助正确识别和划分物种。本研究报道了一个类型为ZW1W2/ZZ的多性别系统，其中雌性2n = 31，雄性2n = 30。检测到携带核仁组织区(NORs)的单对染色体。异染色质位于NOR位点，主要位于染色体的着丝粒区。此外，还获得了大檀香树标本的个体序列COI，共得到3个单倍型。单倍型差异p值在0.2% ~ 7.1%之间。物种划界试验表明，大檀香有两个可能的操作分类单位。因此，本研究报告了裸子目中一个新的多性别系统，这些以前被鉴定为E. affer . desantanai的标本可能属于两个不同的物种。

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.