{"title":"Characterization of Oral Microbiota Following Chemotherapy in Patients With Hematopoietic Malignancies.","authors":"Michi Omori, Nahoko Kato-Kogoe, Shoichi Sakaguchi, Eri Komori, Kazuya Inoue, Kayoko Yamamoto, Wataru Hamada, Tomoyoshi Hayase, Tomoyuki Tano, Shota Nakamura, Takashi Nakano, Hidenori Une, Takaaki Ueno","doi":"10.1177/15347354231159309","DOIUrl":null,"url":null,"abstract":"<p><p>Oral microbiota may be associated with serious local or systemic medical conditions resulting from chemotherapy. This study was conducted to evaluate the changes in the oral microbiota following the initiation of chemotherapy in patients with hematopoietic malignancies and to identify the characteristics of the oral microbiota associated with oral mucositis. Oral samples were collected from 57 patients with hematopoietic malignancies at 2 time points: before the start of chemotherapy and 8 to 20 days after the start of chemotherapy, when chemotherapy-induced oral mucositis often occurs, and 16S rRNA metagenomic analyses were performed. Comparative and linear discriminant analysis effect size (LEfSe) analyses were used to determine the characteristic bacterial groups before and after the initiation of chemotherapy and in those who developed oral mucositis. The alpha and beta diversities of oral microbiota before and after the initiation of chemotherapy differed significantly (operational taxonomic unit index, <i>P</i> < .001; Shannon's index, <i>P</i> < .001; unweighted UniFrac distances, <i>P</i> = .001; and weighted UniFrac distances, <i>P</i> = .001). The LEfSe analysis revealed a group of bacteria whose abundance differed significantly before and after the initiation of chemotherapy. In the group of patients who developed oral mucositis, a characteristic group of bacteria was identified before the start of chemotherapy. In conclusion, we characterized the oral microbiota associated with the initiation of chemotherapy in patients with hematopoietic malignancies. In addition, our findings suggest that oral microbiota composition before the start of chemotherapy may be associated with oral mucositis. The results of this study emphasize the importance of oral management focusing on the oral microbiota during chemotherapy in patients with hematologic malignancies.</p>","PeriodicalId":13734,"journal":{"name":"Integrative Cancer Therapies","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c1/ac/10.1177_15347354231159309.PMC10021090.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Integrative Cancer Therapies","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/15347354231159309","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
引用次数: 0
Abstract
Oral microbiota may be associated with serious local or systemic medical conditions resulting from chemotherapy. This study was conducted to evaluate the changes in the oral microbiota following the initiation of chemotherapy in patients with hematopoietic malignancies and to identify the characteristics of the oral microbiota associated with oral mucositis. Oral samples were collected from 57 patients with hematopoietic malignancies at 2 time points: before the start of chemotherapy and 8 to 20 days after the start of chemotherapy, when chemotherapy-induced oral mucositis often occurs, and 16S rRNA metagenomic analyses were performed. Comparative and linear discriminant analysis effect size (LEfSe) analyses were used to determine the characteristic bacterial groups before and after the initiation of chemotherapy and in those who developed oral mucositis. The alpha and beta diversities of oral microbiota before and after the initiation of chemotherapy differed significantly (operational taxonomic unit index, P < .001; Shannon's index, P < .001; unweighted UniFrac distances, P = .001; and weighted UniFrac distances, P = .001). The LEfSe analysis revealed a group of bacteria whose abundance differed significantly before and after the initiation of chemotherapy. In the group of patients who developed oral mucositis, a characteristic group of bacteria was identified before the start of chemotherapy. In conclusion, we characterized the oral microbiota associated with the initiation of chemotherapy in patients with hematopoietic malignancies. In addition, our findings suggest that oral microbiota composition before the start of chemotherapy may be associated with oral mucositis. The results of this study emphasize the importance of oral management focusing on the oral microbiota during chemotherapy in patients with hematologic malignancies.
口腔微生物群可能与化疗引起的严重局部或全身疾病有关。本研究旨在评估造血恶性肿瘤患者开始化疗后口腔微生物群的变化,并确定与口腔黏膜炎相关的口腔微生物群特征。选取57例造血恶性肿瘤患者,在化疗开始前和化疗开始后8 ~ 20天(化疗引起的口腔黏膜炎常发生)2个时间点采集口腔样本,进行16S rRNA宏基因组分析。采用比较和线性判别分析效应大小(LEfSe)分析确定化疗开始前后和发生口腔黏膜炎患者的特征细菌群。化疗开始前后口腔微生物群α和β多样性差异显著(操作分类单位指数,P P P = .001;加权UniFrac距离,P = .001)。LEfSe分析显示,在化疗开始前后,一组细菌的丰度存在显著差异。在发生口腔黏膜炎的患者组中,在化疗开始前确定了一组特征细菌。总之,我们描述了与造血恶性肿瘤患者开始化疗相关的口腔微生物群。此外,我们的研究结果表明,化疗开始前的口腔微生物群组成可能与口腔黏膜炎有关。本研究的结果强调了在血液恶性肿瘤患者化疗期间口腔微生物群管理的重要性。
期刊介绍:
ICT is the first journal to spearhead and focus on a new and growing movement in cancer treatment. The journal emphasizes scientific understanding of alternative medicine and traditional medicine therapies, and their responsible integration with conventional health care. Integrative care includes therapeutic interventions in diet, lifestyle, exercise, stress care, and nutritional supplements, as well as experimental vaccines, chrono-chemotherapy, and other advanced treatments. Contributors are leading oncologists, researchers, nurses, and health-care professionals.