A comparative study between the possible protective role of melatonin versus its combination with adipose derived-mesenchymal stem cells on experimentally induced diabetic retinopathy in adult male albino rats (Histological and immunohistochemical study).

IF 1.1 4区 医学 Q4 MICROSCOPY Ultrastructural Pathology Pub Date : 2023-05-04 DOI:10.1080/01913123.2023.2184890
Samar Reda, Ghada A Elsammak, Tamer G Elsayed, Samar Abdelaziz Mostafa
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引用次数: 1

Abstract

Diabetes mellitus is a metabolic disorder that can cause numerous ocular issues as well as long-term effects. In our study, we evaluate the effect of melatonin on the diabetic retinal alterations in male albino rats to the effect of melatonin combined with stem cells. 50 adult male rats were equally divided into four groups control, diabetic, melatonin, and melatonin plus stem cells. STZ, 65 mg/kg in phosphate buffered was administered intraperitoneally as a bolus to diabetic group of rats. After inducing diabetes, melatonin (10 mg/kg b.wt./day) was administered orally to the melatonin group for 8 weeks. The stem cell and melatonin group got the same dosage of melatonin as the prior group. They received an intravenous injection of (3?×?106 cell) adipose-derived MSC suspended in phosphate-buffered saline at same time of melatonin ingestion. Animals from all groups had their fundics examined. Following the injection of stem cells, samples of rat retina were collected for light and electron microscopy analyses. H&E and immunohistochemically stained sections revealed a slight improvement in group (III). At the same time, group (IV) results were comparable to those of the control group, which was supported by the findings of an electron microscope. Neovascularization was visible on fundus examination in group (II), whereas it was less noticeable in group (III) and group IV. Melatonin mildly improved the histological structure of the retina in diabetic rats, and when it was combined with adipose-derived MSC, it considerably improved the diabetic alterations.

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褪黑素与其联合脂肪源间充质干细胞对实验性诱导的成年雄性白化大鼠糖尿病视网膜病变可能保护作用的比较研究(组织学和免疫组织化学研究)。
糖尿病是一种代谢紊乱,会引起许多眼部问题和长期影响。在我们的研究中,我们评估了褪黑激素对雄性白化大鼠糖尿病视网膜改变的影响,褪黑激素联合干细胞的作用。50只成年雄性大鼠平均分为四组,对照组、糖尿病组、褪黑素组和褪黑素加干细胞组。STZ, 65 mg/kg磷酸盐缓冲液腹腔内给药给糖尿病组大鼠。诱导糖尿病后,褪黑素组口服褪黑素(10 mg/kg b.w.t. /天),持续8周。干细胞组和褪黑素组得到与前一组相同剂量的褪黑素。他们接受了静脉注射(3 × ×?)(106细胞)脂肪来源的间充质干细胞在摄入褪黑激素的同时悬浮在磷酸盐缓冲盐水中。所有组的动物都进行了基础检查。干细胞注射后,收集大鼠视网膜样本进行光镜和电镜分析。H&E和免疫组织化学染色切片显示(III)组有轻微改善。同时,(IV)组的结果与对照组相当,电镜观察结果也支持这一点。(II)组眼底检查可见新生血管,而(III)组和(IV)组则不太明显。褪黑素轻度改善糖尿病大鼠视网膜的组织学结构,当它与脂肪来源的MSC联合使用时,它显著改善了糖尿病的改变。
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来源期刊
Ultrastructural Pathology
Ultrastructural Pathology 医学-病理学
CiteScore
2.00
自引率
10.00%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.
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