Untargeted metabolomics analysis of serum and follicular fluid samples from women with polycystic ovary syndrome.

IF 2.5 Q3 ENDOCRINOLOGY & METABOLISM Minerva endocrinology Pub Date : 2023-06-01 DOI:10.23736/S2724-6507.20.03199-5
Wan-Li Xu, Guo-Yan Liu, Ning Zhang, Jian Ren, Xiu-Yang Li, Yu-Qiu Li, Yu Chen, Jia-Yi Liu
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Abstract

Background: Polycystic ovary syndrome (PCOS) is a complex endocrine disorder with well-established metabolic abnormalities. In the present study, untargeted metabolomics technology was applied to analyze the serum and follicular fluid samples from women with polycystic ovary syndrome and healthy controls using 1H nuclear magnetic resonance (NMR).

Methods: Seventy samples for PCOS analysis were collected in hospital of Shandong University of Traditional Chinese Medicine (Jinan, China), NMR was used as analytical technology and multivariate analysis was applied to analyze metabolomics difference in PCOS and healthy controls.

Results: Significant metabolic differences were found in both serum and follicular fluid samples with orthogonal partial least-squares discriminant analysis (OPLS-DA). Three discriminated metabolites (1-Methylhistidine, threonine and Citrate) in both serum and follicular fluid were altered in PCOS patients. Abnormal energy metabolism, lipid metabolism and amino acid metabolism were detected in PCOS patients. Furthermore, more significantly changed amino acids were discovered in follicular fluid samples.

Conclusions: Our findings would provide a resource for further investigations on metabolic disturbance in PCOS patients.

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多囊卵巢综合征妇女血清和卵泡液样本的非靶向代谢组学分析
背景:多囊卵巢综合征(PCOS)是一种复杂的内分泌疾病,具有明确的代谢异常。本研究采用非靶向代谢组学技术,利用1H核磁共振(NMR)对多囊卵巢综合征妇女和健康对照者的血清和卵泡液样本进行分析。方法:在山东中医药大学附属医院采集PCOS分析样本70份,采用核磁共振(NMR)作为分析技术,采用多因素分析方法分析PCOS患者与健康对照组代谢组学差异。结果:用正交偏最小二乘判别分析(OPLS-DA)发现血清和卵泡液样品的代谢差异显著。多囊卵巢综合征患者血清和卵泡液中3种特异性代谢物(1-甲基组氨酸、苏氨酸和柠檬酸盐)发生改变。PCOS患者能量代谢、脂质代谢、氨基酸代谢均有异常。此外,在卵泡液样本中发现了更显着变化的氨基酸。结论:本研究为进一步研究PCOS患者的代谢紊乱提供了参考。
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