Biopsy Diagnosis of Hirschsprung's Disease Using a Single Excisional Biopsy Based on the Anorectal Line.

IF 1.5 3区 医学 Q2 PEDIATRICS European Journal of Pediatric Surgery Pub Date : 2024-06-01 Epub Date: 2023-03-30 DOI:10.1055/a-2065-9071
Masahiro Takeda, Takanori Ochi, Hiroyuki Koga, Koichi Tsuboi, Atsushi Arakawa, Geoffrey J Lane, Atsuyuki Yamataka
{"title":"Biopsy Diagnosis of Hirschsprung's Disease Using a Single Excisional Biopsy Based on the Anorectal Line.","authors":"Masahiro Takeda, Takanori Ochi, Hiroyuki Koga, Koichi Tsuboi, Atsushi Arakawa, Geoffrey J Lane, Atsuyuki Yamataka","doi":"10.1055/a-2065-9071","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong> A biopsy protocol for diagnosing Hirschsprung's disease (HD) in children using the anorectal line (ARL).</p><p><strong>Materials and methods: </strong> The ARL was adopted for diagnosing HD in 2016 using two excisional submucosal rectal biopsies performed at different levels, sequentially; the first just above the ARL and the second, further proximal (2-ARL). Currently, only the first-level biopsy is performed (1-ARL) and examined intraoperatively. Management was observation if normoganglionic, pull-through if aganglionic, and a second-level biopsy if hypoganglionic. Hypoganglionosis was considered physiologic if the second-level biopsy was normoganglionic and pathologic if hypoganglionic. Colon caliber change and bowel obstructive symptoms reflect the severity of hypoganglionosis.</p><p><strong>Results: </strong> For 2-ARL (<i>n</i> = 54), results were: normoganglionosis (<i>n</i> = 31/54; 57.4%), aganglionosis (<i>n</i> = 19/54; 35.2%), and hypoganglionosis (<i>n</i> = 4/54; 7.4%); physiologic (<i>n</i> = 3/54; 5.6%) and pathologic (<i>n</i> = 1/54; 1.9%). Normoganglionosis and aganglionosis were always duplicated in 2-ARL (kappa = 1.0). For 1-ARL (<i>n</i> = 36), results were: normoganglionosis (<i>n</i> = 17/36; 47.2%), aganglionosis (<i>n</i> = 17/36; 47.2%), and hypoganglionosis (<i>n</i> = 2/36; 5.6%). Second-level biopsies were normoganglionic (physiologic: <i>n</i> = 1) and hypoganglionic (pathologic: <i>n</i> = 1). All normoganglionic cases, except one, resolved conservatively. All aganglionic cases had pull-through with HD confirmed on histopathology. Both pathologic hypoganglionic cases had caliber change and severe obstructive symptoms as definitive indications for pull-through with hypoganglionosis of the entire rectum confirmed on histopathology. Physiologic hypoganglionic cases were observed and currently have regular defecation.</p><p><strong>Conclusion: </strong> Because the ARL is an objective functional, neurologic, and anatomic demarcation, normoganglionosis and aganglionosis can be diagnosed accurately with a single excisional biopsy. Only hypoganglionosis requires a second-level biopsy.</p>","PeriodicalId":56316,"journal":{"name":"European Journal of Pediatric Surgery","volume":" ","pages":"274-281"},"PeriodicalIF":1.5000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Pediatric Surgery","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1055/a-2065-9071","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/3/30 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PEDIATRICS","Score":null,"Total":0}
引用次数: 0

Abstract

Introduction:  A biopsy protocol for diagnosing Hirschsprung's disease (HD) in children using the anorectal line (ARL).

Materials and methods:  The ARL was adopted for diagnosing HD in 2016 using two excisional submucosal rectal biopsies performed at different levels, sequentially; the first just above the ARL and the second, further proximal (2-ARL). Currently, only the first-level biopsy is performed (1-ARL) and examined intraoperatively. Management was observation if normoganglionic, pull-through if aganglionic, and a second-level biopsy if hypoganglionic. Hypoganglionosis was considered physiologic if the second-level biopsy was normoganglionic and pathologic if hypoganglionic. Colon caliber change and bowel obstructive symptoms reflect the severity of hypoganglionosis.

Results:  For 2-ARL (n = 54), results were: normoganglionosis (n = 31/54; 57.4%), aganglionosis (n = 19/54; 35.2%), and hypoganglionosis (n = 4/54; 7.4%); physiologic (n = 3/54; 5.6%) and pathologic (n = 1/54; 1.9%). Normoganglionosis and aganglionosis were always duplicated in 2-ARL (kappa = 1.0). For 1-ARL (n = 36), results were: normoganglionosis (n = 17/36; 47.2%), aganglionosis (n = 17/36; 47.2%), and hypoganglionosis (n = 2/36; 5.6%). Second-level biopsies were normoganglionic (physiologic: n = 1) and hypoganglionic (pathologic: n = 1). All normoganglionic cases, except one, resolved conservatively. All aganglionic cases had pull-through with HD confirmed on histopathology. Both pathologic hypoganglionic cases had caliber change and severe obstructive symptoms as definitive indications for pull-through with hypoganglionosis of the entire rectum confirmed on histopathology. Physiologic hypoganglionic cases were observed and currently have regular defecation.

Conclusion:  Because the ARL is an objective functional, neurologic, and anatomic demarcation, normoganglionosis and aganglionosis can be diagnosed accurately with a single excisional biopsy. Only hypoganglionosis requires a second-level biopsy.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
利用基于肛门直肠线的单次切除活检诊断赫氏贲门失弛缓症
导言:使用肛门直肠线(ARL)诊断儿童赫氏病(HD)的活检方案:2016年,采用ARL诊断HD,在不同水平依次进行两次直肠黏膜下切除活检;第一次在ARL上方,第二次在更近端(2-ARL)。目前,只进行第一层活检(1-ARL)并在术中进行检查。如果神经节正常,则采用观察法;如果神经节变性,则采用拉通法;如果神经节功能减退,则采用二级活检法。如果二级活检结果为正常神经节,则认为是生理性神经节功能减退;如果是神经节功能减退,则认为是病理性神经节功能减退。结肠口径变化和肠梗阻症状反映了神经根功能减退症的严重程度:对于 2-ARL(n = 54),结果为:正常结肠神经节病(n = 31/54;57.4%)、结肠神经节病(n = 19/54;35.2%)和结肠神经节功能减退(n = 4/54;7.4%);生理性(n = 3/54;5.6%)和病理性(n = 1/54;1.9%)。在 2-ARL 中,正常神经节病变和神经节病变总是重复的(kappa = 1.0)。1-ARL(n = 36)的结果为:正常神经节病变(n = 17/36;47.2%)、神经节病变(n = 17/36;47.2%)和神经节发育不全(n = 2/36;5.6%)。二级活检结果为正常神经节病变(生理学:n = 1)和低神经节病变(病理学:n = 1)。除一个病例外,所有正常神经节病例均可保守治疗。所有激动型病例均有牵拉,组织病理学证实为 HD。两例病理性神经节功能减退病例均以口径改变和严重阻塞症状为明确指征,经组织病理学检查证实为整个直肠神经节功能减退,需要进行牵拉治疗。经观察,生理性直肠神经节功能减退病例目前排便正常:结论:由于 ARL 是一个客观的功能、神经和解剖分界线,因此只需进行一次切除活检,就能准确诊断正常肛门神经节病变和肛门神经节病变。只有神经节发育不全才需要进行二级活检。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
3.90
自引率
5.60%
发文量
66
审稿时长
6-12 weeks
期刊介绍: This broad-based international journal updates you on vital developments in pediatric surgery through original articles, abstracts of the literature, and meeting announcements. You will find state-of-the-art information on: abdominal and thoracic surgery neurosurgery urology gynecology oncology orthopaedics traumatology anesthesiology child pathology embryology morphology Written by surgeons, physicians, anesthesiologists, radiologists, and others involved in the surgical care of neonates, infants, and children, the EJPS is an indispensable resource for all specialists.
期刊最新文献
Anatomical Variations of the External Genitalia in Posterior Cloaca: Clinical Consequences of Misdiagnosis-A Systematic Review of the Literature and the ARM-Net Consortium Experience. Intercostal Nerve Cryoablation or Epidural Analgesia for Multimodal Pain Management after the Nuss Procedure: A Cohort Study. Evaluation of Tubularized Incised Plate Urethroplasty with Spongioplasty-Dartosoraphy Reinforcement in Pediatric Hypospadias: A Randomized Controlled Study. Social Determinants of Health Are Associated with Failed Bowel Management for Children with Anorectal Malformations. Treatment of Anorectal Malformations in German Hospitals: Analysis of National Hospital Discharge Data from 2016 to 2021.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1