Therapeutic and Preventive Effect of Orally Administered Prebiotics on Atopic Dermatitis in a Mouse Model.

IF 4.1 2区 医学 Q2 ALLERGY Allergy, Asthma & Immunology Research Pub Date : 2023-05-01 DOI:10.4168/aair.2023.15.3.303
Minje Kang, Ji-Hye Jung, Ji-Young Kim, Seok-Ho Hong, Young Her
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引用次数: 2

Abstract

Purpose: Recently, interest is increasing in using prebiotics, which are nutrient ingredients of live microorganism that improve the intestinal environments by promoting the growth of beneficial gut microflora. Although numerous studies have demonstrated the beneficial effects of probiotics on atopic dermatitis (AD) development, few have examined preventive and therapeutic effects of prebiotics on the onset and progression of AD.

Methods: In this study, we investigated therapeutic and preventive effect of prebiotics, including β-glucan and inulin, using an oxazolone (OX)-induced AD-like mouse model. Prebiotics were orally administered 2 weeks after the end of sensitization period (therapeutic study) and 3 weeks before the initial sensitization (prevention study). The physiological and histological alterations in the skin and gut of the mice were investigated.

Results: In the therapeutic study, the severity of skin lesions and inflammatory responses were effectively reduced after administering β-glucan and inulin, respectively. The expression level of calprotectin was significantly decreased by approximately 2-fold (P < 0.05) in the skin and gut of prebiotics-treated mice compared to the control. In addition, epidermal thickness and the number of infiltrated immune cells were markedly reduced in the dermis of prebiotics-treated mice compared with to those in the OX-induced mice (P < 0.05). These findings were same as in the prevention study. Importantly, pre-administration of β-glucan and inulin prevented the progression of AD by promoting the growth of good bacteria in the gut of OX-induced AD mice. However, the co-administration of β-glucan and inulin did not show enhanced preventive effects on these alterations.

Conclusions: Prebiotics has a therapeutic effect on AD in OX-induced AD mouse model. Moreover, our study suggests that prebiotics prevents the development of AD and this effect is associated with a change in gut microbiome.

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口服益生元对小鼠特应性皮炎的治疗和预防作用。
目的:近年来,人们对益生元的使用越来越感兴趣,益生元是一种活微生物的营养成分,通过促进有益肠道菌群的生长来改善肠道环境。虽然许多研究已经证明益生菌对AD的发展有益,但很少有人研究益生菌对AD发病和进展的预防和治疗作用。方法:采用恶唑啉酮(OX)诱导的ad样小鼠模型,研究β-葡聚糖和菊粉等益生元的治疗和预防作用。在致敏期结束后2周(治疗研究)和初始致敏前3周(预防研究)口服益生元。观察小鼠皮肤和肠道的生理和组织学变化。结果:在治疗性研究中,分别给予β-葡聚糖和菊粉后,皮肤病变的严重程度和炎症反应均有效减轻。与对照组相比,益生元组小鼠皮肤和肠道中钙保护蛋白的表达水平显著降低约2倍(P < 0.05)。此外,与ox诱导的小鼠相比,益生元处理的小鼠真皮表皮厚度和浸润免疫细胞数量明显减少(P < 0.05)。这些发现与预防研究的结果相同。重要的是,预给药β-葡聚糖和菊糖通过促进ox诱导的AD小鼠肠道中有益菌的生长来阻止AD的进展。然而,β-葡聚糖和菊粉的共同施用并没有显示出对这些改变的增强预防作用。结论:益生元对ox诱导的AD小鼠模型有治疗作用。此外,我们的研究表明,益生元可以预防AD的发展,这种作用与肠道微生物群的变化有关。
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来源期刊
CiteScore
6.10
自引率
6.80%
发文量
53
审稿时长
>12 weeks
期刊介绍: The journal features cutting-edge original research, brief communications, and state-of-the-art reviews in the specialties of allergy, asthma, and immunology, including clinical and experimental studies and instructive case reports. Contemporary reviews summarize information on topics for researchers and physicians in the fields of allergy and immunology. As of January 2017, AAIR do not accept case reports. However, if it is a clinically important case, authors can submit it in the form of letter to the Editor. Editorials and letters to the Editor explore controversial issues and encourage further discussion among physicians dealing with allergy, immunology, pediatric respirology, and related medical fields. AAIR also features topics in practice and management and recent advances in equipment and techniques for clinicians concerned with clinical manifestations of allergies and pediatric respiratory diseases.
期刊最新文献
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