Curcumin alleviated oxidation stress injury by mediating osteopontin in nephrolithiasis rats.

IF 1.1 4区医学 Q3 SURGERY Acta cirurgica brasileira Pub Date : 2023-01-01 DOI:10.1590/acb380223

Jian-Jun Huang, Xu-Ping Yao, Ping Zhang, Zhi-Ling Lou, Hong-Gang Qi, Hou-Meng Yang, Guo-Bin Weng

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Abstract

Purpose: To explore the role and mechanism of curcumin (Cur) in reducing oxidative stress damage in rats with nephrolithiasis induced by ethylene glycol (EG).

Methods: Thirty male rats were divided into normal control, model, positive (10% potassium citrate), Cur-10 (10 mg/kg curcumin) and Cur-20 (20 mg/kg curcumin) groups.

Results: The results of kidney tissue section stained by hematoxylin-eosin and von Kossa showed that curcumin treatment can inhibit the formation of kidney stones. The biochemical test results showed that the urea (Ur), creatinine (Cr), uric acid (UA), inorganic phosphorus and Ca2+ concentrations in urine decreased after being treated with curcumin. There were significant differences between different doses of curcumin (P < 0.05). Compared with the Cur-10 group, Cur-20 had a more significant inhibitory effect on malondialdehyde (MDA) (P < 0.05). In addition, reverse transcription polymerase chain reaction (PCR) detection and immunohistochemical results indicated that the osteopontin (OPN) in the kidney was significantly reduced after curcumin treatment.

Conclusions: Curcumin could reduce the oxidative stress damage caused by EG-induced kidney stones.

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姜黄素通过介导骨桥蛋白减轻肾结石大鼠氧化应激损伤。

目的:探讨姜黄素(curcumin, Cur)减轻乙二醇所致肾结石大鼠氧化应激损伤的作用及机制。方法:将30只雄性大鼠分为正常对照组、模型组、阳性组(10%柠檬酸钾)、Cur-10组(10 mg/kg姜黄素)和Cur-20组(20 mg/kg姜黄素)。结果:苏木精-伊红和von Kossa染色肾组织切片显示姜黄素处理能抑制肾结石的形成。生化试验结果表明，姜黄素处理后，尿中尿素(Ur)、肌酐(Cr)、尿酸(UA)、无机磷和Ca2+浓度降低。姜黄素不同剂量间差异有统计学意义(P < 0.05)。与cu -10组相比，cu -20对丙二醛(MDA)的抑制作用更显著(P < 0.05)。此外，逆转录聚合酶链反应(PCR)检测和免疫组化结果显示姜黄素治疗后肾脏骨桥蛋白(OPN)明显降低。结论:姜黄素可减轻eg所致肾结石的氧化应激损伤。

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