Metagenomic next-generation sequencing for detection of pathogens in children with hematological diseases complicated with infection

IF 2.3 3区生物学 Q3 BIOCHEMICAL RESEARCH METHODS Molecular and Cellular Probes Pub Date : 2023-02-01 DOI:10.1016/j.mcp.2022.101889

Yating Zhang , Dunhua Zhou , Han Xia , Jian Wang , Huaqing Yang , Luhong Xu , Ke Huang , Jianpei Fang

{"title":"Metagenomic next-generation sequencing for detection of pathogens in children with hematological diseases complicated with infection","authors":"Yating Zhang , Dunhua Zhou , Han Xia , Jian Wang , Huaqing Yang , Luhong Xu , Ke Huang , Jianpei Fang","doi":"10.1016/j.mcp.2022.101889","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>Infection is one of the most common causes of death in children with hematological diseases. Here, we aim to investigate the value of metagenomic next-generation sequencing (mNGS) in the detection of causative pathogens in children with hematological diseases.</p></div><div><h3>Methods</h3><p>In this retrospective study, specimens from children with hematological diseases, who were admitted to Sun Yat-Sen University between June 2019 and September 2021, were collected for culture and mNGS.</p></div><div><h3>Results</h3><p>A total of 67 pediatric patients were enrolled, and 96 specimens were collected. The positive rate of mNGS was significantly higher than that of culture (57.2% vs 12.5%, <em>P</em> < 0.01). The concordance (90.9%, 10/11) between the positive results of the two methods was high. mNGS detected more cases with <em>Pneumocystis jeroveci</em>, <em>Aspergillus flavus</em>, viruses, and some rare pathogens than culture. Mixed infections were detected by mNGS in 16 cases. Clinical anti-infective treatment was adjusted according to the results of mNGS, the conditions of most patients improved.</p></div><div><h3>Conclusion</h3><p>Compared to culture, mNGS shows great advantages in diagnosing bacterial, fungal, viral, and mixed infections in children with hematologic diseases, positively impacting clinical care. mNGS can be used as a complement to culture for pathogen detection.</p></div>","PeriodicalId":49799,"journal":{"name":"Molecular and Cellular Probes","volume":"67 ","pages":"Article 101889"},"PeriodicalIF":2.3000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular and Cellular Probes","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0890850822001001","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 2

Abstract

Objective

Infection is one of the most common causes of death in children with hematological diseases. Here, we aim to investigate the value of metagenomic next-generation sequencing (mNGS) in the detection of causative pathogens in children with hematological diseases.

Methods

In this retrospective study, specimens from children with hematological diseases, who were admitted to Sun Yat-Sen University between June 2019 and September 2021, were collected for culture and mNGS.

Results

A total of 67 pediatric patients were enrolled, and 96 specimens were collected. The positive rate of mNGS was significantly higher than that of culture (57.2% vs 12.5%, P < 0.01). The concordance (90.9%, 10/11) between the positive results of the two methods was high. mNGS detected more cases with Pneumocystis jeroveci, Aspergillus flavus, viruses, and some rare pathogens than culture. Mixed infections were detected by mNGS in 16 cases. Clinical anti-infective treatment was adjusted according to the results of mNGS, the conditions of most patients improved.

Conclusion

Compared to culture, mNGS shows great advantages in diagnosing bacterial, fungal, viral, and mixed infections in children with hematologic diseases, positively impacting clinical care. mNGS can be used as a complement to culture for pathogen detection.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

宏基因组下一代测序用于检测儿童血液系统疾病并发感染的病原体

感染是儿童血液系统疾病最常见的死亡原因之一。在这里，我们的目的是研究宏基因组下一代测序（mNGS）在检测血液病儿童致病病原体方面的价值。方法在这项回顾性研究中，收集2019年6月至2021年9月期间入住中山大学的血液病儿童的样本进行培养和mNGS。结果共有67名儿科患者入选，共收集96份样本。mNGS的阳性率明显高于培养（57.2%vs12.5%，P<；0.01），两种方法的阳性结果一致性高（90.9%，10/11）。mNGS检测到的jeroveci肺孢子虫、黄曲霉、病毒和一些罕见病原体的病例比培养的多。mNGS检测到16例混合感染。临床抗感染治疗根据mNGS的结果进行调整，大多数患者的病情有所改善。结论与培养相比，mNGS在诊断儿童血液系统疾病的细菌、真菌、病毒和混合感染方面具有很大优势，对临床护理有积极影响。mNGS可作为培养物的补充用于病原体检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Molecular and Cellular Probes 生物-生化研究方法

CiteScore

6.80

自引率

0.00%

发文量

审稿时长

16 days

期刊介绍： MCP - Advancing biology throughâ€“omics and bioinformatic technologies wants to capture outcomes from the current revolution in molecular technologies and sciences. The journal has broadened its scope and embraces any high quality research papers, reviews and opinions in areas including, but not limited to, molecular biology, cell biology, biochemistry, immunology, physiology, epidemiology, ecology, virology, microbiology, parasitology, genetics, evolutionary biology, genomics (including metagenomics), bioinformatics, proteomics, metabolomics, glycomics, and lipidomics. Submissions with a technology-driven focus on understanding normal biological or disease processes as well as conceptual advances and paradigm shifts are particularly encouraged. The Editors welcome fundamental or applied research areas; pre-submission enquiries about advanced draft manuscripts are welcomed. Top quality research and manuscripts will be fast-tracked.