Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells

IF 4.7 Q2 IMMUNOLOGY Journal of Translational Autoimmunity Pub Date : 2023-01-01 DOI:10.1016/j.jtauto.2023.100202
Markos Patsouras , Eirini Alexopoulou , Spyros Foutadakis , Eirini Tsiki , Panagiota Karagianni , Marios Agelopoulos , Panayiotis G. Vlachoyiannopoulos
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Abstract

Antiphospholipid syndrome (APS) is an autoimmune thrombophilia characterized by recurrent thrombotic events and/or pregnancy morbidity in the presence of antiphospholipid antibodies detected either as anti-cardiolipin, anti-β2 Glycoprotein I (anti-β2GPI) or Lupus anticoagulant (LA). Endothelial deregulation characterizes the syndrome. To address gene expression changes accompanying the development of autoimmune phenotype in endothelial cells in the context of APS, we performed transcriptomics analysis in Human Umbilical Vein Endothelial Cells (HUVECs) stimulated with IgG from APS patients and β2GPI, followed by intersection of RNA-seq data with published microarray and ChIP-seq results (Chromatin Immunoprecipitation). Our strategy revealed that during HUVEC activation diverse signaling pathways such as TNF-α, TGF-β, MAPK38, and Hippo are triggered as indicated by Gene Ontology (GO) classification and pathway analysis. Finally, cell biology approaches performed side-by-side in naïve and stimulated cultured HUVECs, as well as, in placenta specimens derived from Healthy donors (HDs) and APS-patients verified the evolution of an APS-characteristic gene expression program in endothelial cells during the initial stages of the disease's development.

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抗磷脂抗体诱导内皮细胞的促炎和促凝途径
抗磷脂综合征(APS)是一种自身免疫性血栓形成倾向性疾病,其特征是在存在抗磷脂抗体(抗心磷脂、抗β2糖蛋白I(抗β2GPI)或狼疮抗凝剂(LA))的情况下反复发生血栓事件和/或妊娠发病率。内皮失调是该综合征的特征。为了解决APS背景下内皮细胞中伴随自身免疫表型发展的基因表达变化,我们对用APS患者IgG和β2GPI刺激的人脐静脉内皮细胞(HUVECs)进行了转录组学分析,然后将RNA-seq数据与已发表的微阵列和ChIP-seq结果进行交叉(染色质免疫沉淀)。我们的策略显示,在HUVEC激活过程中,不同的信号通路,如TNF-α、TGF-β、MAPK38和Hippo,如基因本体论(GO)分类和通路分析所示,会被触发。最后,在幼稚和刺激培养的HUVECs中,以及在来自健康供体(HD)和APS患者的胎盘标本中并行进行的细胞生物学方法验证了在疾病发展的初始阶段内皮细胞中APS特征基因表达程序的进化。
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来源期刊
Journal of Translational Autoimmunity
Journal of Translational Autoimmunity Medicine-Immunology and Allergy
CiteScore
7.80
自引率
2.60%
发文量
33
审稿时长
55 days
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