{"title":"Relationship among seminal antigenicity, antioxidant status and metabolically active sperm from Holstein-Friesian (<i>Bos taurus</i>) bulls.","authors":"Santhanahalli Siddalingappa Archana, Divakar Swathi, Laxman Ramya, Hulliyurdurga Shameeulla Heena, Balaganur Krishnappa, Bala Krishnan Binsila, Duraisamy Rajendran, Sellappan Selvaraju","doi":"10.1080/19396368.2023.2198070","DOIUrl":null,"url":null,"abstract":"<p><p>Sperm antigenicity has been implicated as a regulatory factor for acquiring fertilizing competence in the female reproductive tract. Overt immune response against the sperm proteins leads to idiopathic infertility. Hence, the aim of the study was to evaluate the influence of the auto-antigenic potential of sperm on the antioxidant status, metabolic activities and reactive oxygen species (ROS) in bovine. Semen from Holstein-Friesian bulls (<i>n</i> = 15) was collected and classified into higher (HA, <i>n</i> = 8) and lower (LA, <i>n</i> = 7) antigenic groups based on micro-titer agglutination assay. The neat semen was subjected to the evaluation of bacterial load, leukocyte count, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and lipid peroxidation (LPO) levels. Antioxidant activities in seminal plasma and intracellular ROS levels in the post-thawed sperm were estimated. The number of leukocytes was lower (<i>p</i> < .05) in the HA than the LA semen. The percentage of metabolically active sperm was higher (<i>p</i> < .05) in HA than the LA group. The activities of total non-enzymatic antioxidant, superoxide dismutase (SOD) and catalase (CAT) were higher (<i>p</i> < .05) while glutathione peroxidase activity was lower (<i>p</i> < .05) in the seminal plasma of LA group. The LPO levels of neat sperm and the percentage sperm positive for intracellular ROS in the cryopreserved sample were lower (<i>p</i> < .05) in the HA group. Auto-antigenic levels were positively correlated with the percentage of metabolically active sperm (<i>r</i> = 0.73, <i>p</i> < .01). However, the seminal auto-antigenicity was negatively (<i>p</i> < .05) correlated with the levels of SOD (<i>r</i>=-0.66), CAT (<i>r</i>=-0.72), LPO (<i>r</i>=-0.602) and intracellular ROS (<i>r</i>=-0.835). The findings were represented in graphical abstract. It is inferred that the higher auto-antigenic levels protect the quality of bovine semen by promoting sperm metabolism and lowering ROS and LPO levels.</p>","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":" ","pages":"366-378"},"PeriodicalIF":2.1000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Systems Biology in Reproductive Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/19396368.2023.2198070","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/5/24 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ANDROLOGY","Score":null,"Total":0}
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Abstract
Sperm antigenicity has been implicated as a regulatory factor for acquiring fertilizing competence in the female reproductive tract. Overt immune response against the sperm proteins leads to idiopathic infertility. Hence, the aim of the study was to evaluate the influence of the auto-antigenic potential of sperm on the antioxidant status, metabolic activities and reactive oxygen species (ROS) in bovine. Semen from Holstein-Friesian bulls (n = 15) was collected and classified into higher (HA, n = 8) and lower (LA, n = 7) antigenic groups based on micro-titer agglutination assay. The neat semen was subjected to the evaluation of bacterial load, leukocyte count, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and lipid peroxidation (LPO) levels. Antioxidant activities in seminal plasma and intracellular ROS levels in the post-thawed sperm were estimated. The number of leukocytes was lower (p < .05) in the HA than the LA semen. The percentage of metabolically active sperm was higher (p < .05) in HA than the LA group. The activities of total non-enzymatic antioxidant, superoxide dismutase (SOD) and catalase (CAT) were higher (p < .05) while glutathione peroxidase activity was lower (p < .05) in the seminal plasma of LA group. The LPO levels of neat sperm and the percentage sperm positive for intracellular ROS in the cryopreserved sample were lower (p < .05) in the HA group. Auto-antigenic levels were positively correlated with the percentage of metabolically active sperm (r = 0.73, p < .01). However, the seminal auto-antigenicity was negatively (p < .05) correlated with the levels of SOD (r=-0.66), CAT (r=-0.72), LPO (r=-0.602) and intracellular ROS (r=-0.835). The findings were represented in graphical abstract. It is inferred that the higher auto-antigenic levels protect the quality of bovine semen by promoting sperm metabolism and lowering ROS and LPO levels.
精子抗原性被认为是女性生殖道获得受精能力的调节因素。对精子蛋白的过度免疫反应会导致特发性不孕。因此，本研究的目的是评估精子自身抗原潜力对牛抗氧化状态、代谢活性和活性氧（ROS）的影响。荷斯坦-弗里斯公牛精液（n = 15） 收集并分类为较高（HA = 8） 和更低（LA，n = 7） 基于微滴度凝集试验的抗原群。对纯精液进行细菌载量、白细胞计数、3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）测定和脂质过氧化（LPO）水平的评估。估计精浆中的抗氧化活性和解冻后精子中的细胞内ROS水平。白细胞数量较低（p p p p p r = 0.73，p p r=-0.66）、CAT（r=-0.72）、LPO（r=-0.602）和细胞内ROS（r=-0.835）。推测较高的自身抗原水平通过促进精子代谢和降低ROS和LPO水平来保护牛精液的质量。
期刊介绍：
Systems Biology in Reproductive Medicine, SBiRM, publishes Research Articles, Communications, Applications Notes that include protocols a Clinical Corner that includes case reports, Review Articles and Hypotheses and Letters to the Editor on human and animal reproduction. The journal will highlight the use of systems approaches including genomic, cellular, proteomic, metabolomic, bioinformatic, molecular, and biochemical, to address fundamental questions in reproductive biology, reproductive medicine, and translational research. The journal publishes research involving human and animal gametes, stem cells, developmental biology and toxicology, and clinical care in reproductive medicine. Specific areas of interest to the journal include: male factor infertility and germ cell biology, reproductive technologies (gamete micro-manipulation and cryopreservation, in vitro fertilization/embryo transfer (IVF/ET) and contraception. Research that is directed towards developing new or enhanced technologies for clinical medicine or scientific research in reproduction is of significant interest to the journal.
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