Effect of Agmatine on a mouse model of allergic airway inflammation: A comparative study.

IF 3.3 4区 医学 Q3 IMMUNOLOGY Autoimmunity Pub Date : 2022-12-01 DOI:10.1080/08916934.2022.2093864
Mohammed K Еlmahdy, Rania R Abdelaziz, Hoda S Elmahdi, Ghada M Suddеk
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Abstract

Introduction: Asthma is a chronic lung disease that injures and constricts the airways. This study evaluates the effects of agmatine on ovalbumin (OVA)-induced allergic inflammation of the airways.

Methods: OVA sensitization by intraperitoneal injection was used to induce airway inflammation in mice on days 0 and 7; then the mice were challenged using beclomethasone (150 µg/kg, inhalation), a standard anti-asthmatic drug, from day 14 to day 16. Furthermore, agmatine (200 mg/kg) was intraperitoneally injected on day 0 and then daily for 16 days, followed by OVA challenge. The lung weight ratio, total and differential cell counts, TNF-α, interleukin-5 (IL-5) and IL-13 in bronchoalveolar lavage fluid (BALF), lung nitrite/nitrate (NO), and oxidative parameters were determined. Moreover, histopathological and immunohistochemical staining was employed.

Results: Injection of agmatine (200 mg/kg) for 16 days significantly attenuated inflammation of the airways. The levels of BALF inflammatory cells, TNF-α, IL-5, IL-13, lung NO, and malondialdehyde (MDA), significantly decreased with concomitant elevation of superoxide dismutase (SOD) levels. Histological and immunohistochemical analyses of mast cells paralleled to biochemical improvements.

Conclusion: Finally, this study illustrated that agmatine attenuates the allergic inflammation of airways caused by OVA by mitigating cytokines release, NO expression, and oxidative stress.

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胍丁胺对小鼠变应性气道炎症模型影响的比较研究。
简介:哮喘是一种慢性肺部疾病,损害和收缩气道。本研究评估了胍丁氨酸对卵清蛋白(OVA)诱导的气道变应性炎症的影响。方法:采用腹腔注射OVA致敏法诱导小鼠气道炎症,时间分别为第0、7天;然后从第14天至第16天给小鼠注射标准抗哮喘药物倍氯米松(150µg/kg,吸入)。在第0天腹腔注射胍丁胺(200 mg/kg),然后每天注射,持续16天,然后进行卵细胞激发。测定肺重比、总细胞数和分化细胞数、支气管肺泡灌洗液(BALF)中TNF-α、白细胞介素-5 (IL-5)和IL-13、肺亚硝酸盐/硝酸盐(NO)及氧化参数。采用组织病理学和免疫组织化学染色。结果:胍丁胺(200mg /kg)连续注射16 d可明显减轻气道炎症反应。BALF炎症细胞、TNF-α、IL-5、IL-13、肺NO和丙二醛(MDA)水平随超氧化物歧化酶(SOD)水平升高而显著降低。肥大细胞的组织学和免疫组织化学分析与生化改善平行。结论:最后,本研究表明,agmatine通过减轻细胞因子释放、NO表达和氧化应激来减轻OVA引起的气道变应性炎症。
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来源期刊
Autoimmunity
Autoimmunity 医学-免疫学
CiteScore
5.70
自引率
8.60%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Autoimmunity is an international, peer reviewed journal that publishes articles on cell and molecular immunology, immunogenetics, molecular biology and autoimmunity. Current understanding of immunity and autoimmunity is being furthered by the progress in new molecular sciences that has recently been little short of spectacular. In addition to the basic elements and mechanisms of the immune system, Autoimmunity is interested in the cellular and molecular processes associated with systemic lupus erythematosus, rheumatoid arthritis, Sjogren syndrome, type I diabetes, multiple sclerosis and other systemic and organ-specific autoimmune disorders. The journal reflects the immunology areas where scientific progress is most rapid. It is a valuable tool to basic and translational researchers in cell biology, genetics and molecular biology of immunity and autoimmunity.
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