Challenges of in situ hybridization in miRNA analysis of triple-negative breast cancer morphological diversity.

IF 2 4区 医学 Q3 ONCOLOGY Neoplasma Pub Date : 2023-04-01 DOI:10.4149/neo_2023_220519N533
Markéta Kolečková, Michala Bezděková, Jan Bouchal, Mária Janíková, Júlia Bohošová, Ondřej Slabý, Marek Svoboda, Josef Srovnal, Katherine Vomáčková, Ivo Überall, Zdeněk Kolář
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Abstract

miRNA expression in triple-negative breast cancers (TNBC) has mainly been studied from a methodological viewpoint. However, it has not been considered that miRNA expression profile may be associated with a specific morphological entity inside every tumor. The verification of this hypothesis on a set of 25 TNBCs was the subject of our previous work, where we confirmed specific expression of the studied miRNAs in 82 samples of different morphologies including inflammatory infiltrate, spindle cell, clear cell, and metastases after RNA extraction and purification as well as microchip and biostatistical analysis. In the current work, we demonstrate a low suitability of in situ hybridization method for miRNA detection compared to RT-qPCR, and in detail discuss the biological role of 8 miRNAs with the most significant changes of expression.

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原位杂交在三阴性乳腺癌形态多样性miRNA分析中的挑战。
miRNA在三阴性乳腺癌(TNBC)中的表达主要是从方法学的角度来研究的。然而,尚未考虑到miRNA表达谱可能与每种肿瘤中的特定形态实体相关。在一组25个tnbc上验证这一假设是我们之前工作的主题,我们在RNA提取和纯化以及微芯片和生物统计学分析后,在82个不同形态的样本中(包括炎症浸润、梭形细胞、透明细胞和转移)证实了所研究的mirna的特异性表达。在目前的工作中,我们论证了原位杂交方法相对于RT-qPCR检测miRNA的适用性较低,并详细讨论了表达变化最显著的8种miRNA的生物学作用。
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来源期刊
Neoplasma
Neoplasma 医学-肿瘤学
CiteScore
5.40
自引率
0.00%
发文量
238
审稿时长
3 months
期刊介绍: The journal Neoplasma publishes articles on experimental and clinical oncology and cancer epidemiology.
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