[Interleukin 34 (IL-34) promotes the proliferation and odontogenic differentiation of rat apical papillary stem cells].

Abstract

Objective To investigate the effect of interleukin-34 (IL-34) on the odontogenic and osteogenic differentiation of stem cells from the apical papilla (SCAPs) in rats. Methods SCAPs were isolated and cultured by enzyme digestion method, and the expression of IL-34 in SCAPs was detected by real-time fluorescence quantitative PCR(RT-PCR). MTT assay was used to analyze the effects of different concentrations of IL-34 on SCAPs' proliferation in rats. The mineralization was observed by alizarin red staining, and the proliferation capacity was detected by scratch test. The expressions of alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP), runt-related transcription factor 2 (Runx2) and critical transcription factor osterix (OSX) were detected by RT-PCR. The protein expressions of ALP, DSPP, Runx2 and OSX were detected by Western blot analysis. Results The maximum concentration of IL-34 promoting the proliferation of SCAPs in rats was 100 ng/mL. Aalizarin red staining showed that IL-34 could promote the mineralization of SCAPs. RT-PCR and Western blot analysis showed that 100 ng/mL IL-34 could promote the expression of ALP, DSPP, Runx2 and OSX. Conclusion IL-34 can promote the proliferation and odontogenic/osteogenic differentiation of SCAPs in rats.