{"title":"A Guide to Flow Cytometry: Components, Basic Principles, Experimental Design, and Cancer Research Applications","authors":"Layal El-Hajjar, Fatima Ali Ahmad, Rihab Nasr","doi":"10.1002/cpz1.721","DOIUrl":null,"url":null,"abstract":"<p>Flow cytometry (FCM) is a state-of-the-art technique for the qualitative and quantitative assessment of cells and other particles’ physical and biological properties. These cells are suspended within a high-velocity fluid stream and pass through a laser beam in single file. The main principle of the FCM instrument is the light scattering and fluorescence emission upon the interaction of the fluorescent particle with the laser beam. It also allows for the physical sorting of particles depending on different parameters. A flow cytometer comprises different components, including fluidic, optics, and electronics systems. This article briefly explains the mechanism of all components of a flow cytometer to clarify the FCM technique's general principles, provides some useful guidelines for the proper design of FCM panels, and highlights some general applications and important applications in cancer research. © 2023 Wiley Periodicals LLC.</p>","PeriodicalId":93970,"journal":{"name":"Current protocols","volume":"3 3","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpz1.721","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Flow cytometry (FCM) is a state-of-the-art technique for the qualitative and quantitative assessment of cells and other particles’ physical and biological properties. These cells are suspended within a high-velocity fluid stream and pass through a laser beam in single file. The main principle of the FCM instrument is the light scattering and fluorescence emission upon the interaction of the fluorescent particle with the laser beam. It also allows for the physical sorting of particles depending on different parameters. A flow cytometer comprises different components, including fluidic, optics, and electronics systems. This article briefly explains the mechanism of all components of a flow cytometer to clarify the FCM technique's general principles, provides some useful guidelines for the proper design of FCM panels, and highlights some general applications and important applications in cancer research. © 2023 Wiley Periodicals LLC.
流式细胞术指南:组成,基本原理,实验设计和癌症研究应用
流式细胞术(FCM)是一种最先进的技术,用于对细胞和其他颗粒的物理和生物特性进行定性和定量评估。这些细胞悬浮在高速流体流中,并以单个文件的形式通过激光束。FCM仪器的主要原理是荧光粒子与激光束相互作用时的光散射和荧光发射。它还允许根据不同的参数对粒子进行物理排序。流式细胞仪包括不同的组件,包括流体、光学和电子系统。本文简要介绍了流式细胞仪各组成部分的作用机制,阐明了FCM技术的一般原理,为正确设计FCM板提供了一些有用的指导,并重点介绍了在癌症研究中的一些一般应用和重要应用。©2023威利期刊有限责任公司。
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