A Human Cell-based Assay to Assess the Induction of Vasculature Formation for Non-genotoxic Carcinogenicity Testing Purposes: A Pilot Study.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-05-01 DOI:10.1177/02611929231171165

Veera Hautanen, Tarja Toimela, Martin Paparella, Tuula Heinonen

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引用次数: 1

Abstract

The induction of vasculature formation is proposed to be a significant mechanism behind the non-genotoxic carcinogenicity of a chemical. The vasculature formation model used in this study is based on the coculture of human primary HUVECs and hASCs. This model was used to develop an assay to assess the induction of vasculature formation. Three assay protocols, based on different conditions, were developed and compared in order to identify the optimal conditions required. Some serum supplements and growth factors were observed to be essential for initiating vasculature formation. Of the studied putative positive reference chemicals, aspartame, sodium nitrite, bisphenol A and nicotine treatment led to a clear induction of vasculature formation, but arsenic and cadmium treatment only led to a slight increase. This human cell-based assay has the potential to be used as one test within a next generation testing battery, to assess the non-genotoxic carcinogenicity of a chemical through the mechanism of vasculature formation induction.

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一项基于人类细胞的试验，用于评估非基因毒性致癌性检测目的的血管形成诱导:一项初步研究。

血管形成的诱导被认为是一种化学物质的非遗传毒性致癌性背后的重要机制。本研究中使用的血管形成模型是基于人原代HUVECs和hASCs的共培养。该模型用于开发一种评估诱导血管形成的试验。根据不同的条件，制定了三种测定方案，并进行了比较，以确定所需的最佳条件。一些血清补充物和生长因子被观察到是开始血管形成所必需的。在所研究的推定阳性对照化学物质中，阿斯巴甜、亚硝酸钠、双酚A和尼古丁处理明显诱导血管形成，而砷和镉处理仅导致血管形成略有增加。这种基于人类细胞的检测方法有潜力作为下一代检测方法中的一种，通过诱导血管形成的机制来评估化学物质的非遗传毒性致癌性。

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