Diagnostic significance of hsa_circ_0000146 and hsa_circ_0000072 biomarkers for Diabetic Kidney Disease in patients with type 2 diabetes mellitus.

IF 2 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Medical Biochemistry Pub Date : 2023-03-15 DOI:10.5937/jomb0-39361

Amul M Badr, Omayma Elkholy, Mona Said, Sally A Fahim, Mohamed El-Khatib, Dina Sabry, Radwa M Gaber

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Abstract

Background: Diabetic Kidney Disease (DKD) is a significant challenge in healthcare. However, there are currently no reliable biomarkers for renal impairment diagnosis, prognosis, or staging in DKD patients. CircRNAs and microRNAs have emerged as noninvasive and efficient biomarkers.

Methods: We explored Cannabinoid receptor 1 (CNR1), C reactive protein (CRP), hsa_circ_ 0000146 and 0000072, and hsa-miR-21 and 495 as diagnostic biomarkers in DKD. The serum concentrations of CRP and CNR1 were measured using ELISA. Rt-qPCR was used to evaluate the expression levels of CNR1, circRNAs, and miRNAs in 55 controls, 55 type 2 diabetes mellitus patients, and 55 DKD patients. Their diagnostic value was determined by their ROC curve. KEGG pathway was used to predict the functional mechanism of the circRNA's target genes.

Results: DKD patients exhibited a significant increase in CRP and CNR1 levels and the expression of miR-21 and 495. The expression levels of circ_0000146 and 0000072 decreased in DKD patients. ROC analysis revealed that circRNAs and miRNAs alone or CNR1 and CRP have significant diagnostic potential. The functional prediction results showed the involvement of hsa_circ_0000146 and 0000072 in various pathways that regulate DKD.

Conclusions: Therefore, the examined circRNAs and miRNAs may represent a novel noninvasive biomarker for diagnosing and staging DKD.

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hsa_circ_0000146和hsa_circ_0000072生物标志物对2型糖尿病患者糖尿病肾病的诊断意义

背景:糖尿病肾病(DKD)是医疗保健领域的一个重大挑战。然而，目前还没有可靠的生物标志物用于DKD患者的肾脏损害诊断、预后或分期。circrna和microrna已成为无创和有效的生物标志物。方法:我们探索大麻素受体1 (CNR1)、C反应蛋白(CRP)、hsa_circ_ 0000146和0000072、hsa-miR-21和495作为DKD的诊断生物标志物。采用ELISA法检测血清CRP和CNR1浓度。采用Rt-qPCR技术评估55例对照组、55例2型糖尿病患者和55例DKD患者中CNR1、circRNAs和miRNAs的表达水平。其诊断价值由ROC曲线决定。KEGG通路用于预测circRNA靶基因的功能机制。结果:DKD患者CRP、CNR1水平显著升高，miR-21、495表达显著升高。circ_0000146和0000072在DKD患者中表达水平降低。ROC分析显示circRNAs和miRNAs单独或CNR1和CRP具有显著的诊断潜力。功能预测结果显示hsa_circ_0000146和0000072参与调节DKD的多种途径。结论:因此，检测的circrna和mirna可能代表一种新的无创生物标志物，用于诊断和分期DKD。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Medical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.00

自引率

12.00%

发文量

审稿时长

>12 weeks

期刊介绍： The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly. The Journal publishes original scientific and specialized articles on all aspects of clinical and medical biochemistry, molecular medicine, clinical hematology and coagulation, clinical immunology and autoimmunity, clinical microbiology, virology, clinical genomics and molecular biology, genetic epidemiology, drug measurement, evaluation of diagnostic markers, new reagents and laboratory equipment, reference materials and methods, reference values, laboratory organization, automation, quality control, clinical metrology, all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.