Preparation and Characterization of Crayfish (Astacus leptodactylus) Chitosan with Different Deacetylation Degrees.

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Iranian Journal of Biotechnology Pub Date : 2023-04-01 DOI:10.30498/ijb.2023.323958.3253
Ali Eslem Kadak, Aygül Küçükgülmez, Mehmet Çelik
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引用次数: 1

Abstract

Background: In this study, chitosan with various deacetylation degrees was extracted from crayfish (Astacus leptodactylus) shells with the purpose of examining the effect of deacetylation on the characterization of chitosan.

Objectives: Recycling of wastes has become an important issue with the advancement of shellfish processing technology. Therefore, this study examined the most important and conventional characterization parameters of chitosan extracted from crayfish shells and investigated whether crayfish chitosan can be an alternative to commercial products.

Material and methods: In order to determine the characterization of the chitosan; degree of deacetylation, yield, molecular weight, apparent viscosity, water binding capacity, fat binding capacity, moisture content, ash content, color properties, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and X-ray diffraction analyses (XRD) were applied.

Results: The low (LDD) and high (HDD) deacetylated crayfish chitosan characterization results in terms of yield, molecular weight, apparent viscosity, water binding capacity, fat binding capacity, moisture content, ash content were 17.50%, 424.03-334.66 kDa, 16.82-9.63 cP, 481.29-428.04%, 419.30-355.75%, 3.32-1.03%, 0.98-1.01%, respectively. As detected by two different methods, potentiometric titration and elemental analysis, the deacetylation degrees of low and high crayfish chitosan were found close to each other, which were 76.98-94.98% and 73.79-92.06%, respectively. As the deacetylation period extended, acetyl groups were removed, and the degree of the deacetylation of crayfish chitosan increased while the apparent viscosity, molecular weight, water and fat binding capacity decreased.

Conclusions: The findings of the present study are important to obtain the chitosan having various physicochemical characteristics from unevaluated crayfish wastes and to use it in many different sectors, especially biotechnology, medicine, pharmaceutical, food, and agriculture.

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不同脱乙酰度小龙虾壳聚糖的制备与表征
背景:本研究从小龙虾壳中提取不同脱乙酰度的壳聚糖,考察脱乙酰对壳聚糖表征的影响。目的:随着贝类加工技术的进步,废物的回收利用已成为一个重要问题。因此,本研究考察了从小龙虾壳中提取壳聚糖的最重要和最常规的表征参数,并探讨了小龙虾壳聚糖是否可以作为商业产品的替代品。材料和方法:为了确定壳聚糖的表征;采用脱乙酰化程度、收率、分子量、表观粘度、水结合力、脂肪结合力、水分含量、灰分含量、颜色性能、傅里叶变换红外光谱(FT-IR)、扫描电镜(SEM)和x射线衍射分析(XRD)进行了表征。结果:低(LDD)和高(HDD)脱乙酰小龙虾壳聚糖的产率、分子量、表观粘度、水结稠度、脂肪结稠度、水分含量、灰分含量分别为17.50%、424.03 ~ 334.66 kDa、16.82 ~ 9.63 cP、481.29 ~ 428.04%、419.30 ~ 355.75%、3.32 ~ 1.03%、0.98 ~ 1.01%。通过电位滴定法和元素分析两种不同的检测方法,发现低、高螯虾壳聚糖的脱乙酰度接近,分别为76.98 ~ 94.98%和73.79 ~ 92.06%。随着脱乙酰期的延长,乙酰基被去除,小龙虾壳聚糖的脱乙酰程度增加,表观粘度、分子量、水和脂肪结合能力下降。结论:本研究结果对从未评价过的小龙虾废弃物中提取具有多种理化特性的壳聚糖,并将其应用于生物技术、医药、食品和农业等领域具有重要意义。
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来源期刊
Iranian Journal of Biotechnology
Iranian Journal of Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
2.60
自引率
7.70%
发文量
20
期刊介绍: Iranian Journal of Biotechnology (IJB) is published quarterly by the National Institute of Genetic Engineering and Biotechnology. IJB publishes original scientific research papers in the broad area of Biotechnology such as, Agriculture, Animal and Marine Sciences, Basic Sciences, Bioinformatics, Biosafety and Bioethics, Environment, Industry and Mining and Medical Sciences.
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