NOTCH1- and CD117-positive stem cells in human endometrium and their implications for successful implantation

Margarita Ruseva M.Sc. , Dimitar Parvanov Ph.D. , Rumiana Ganeva M.Sc. , Maria Handzhiyska B.Sc. , Nina Vidolova M.Sc. , Dimitar Metodiev M.D. , Georgi Stamenov M.D., Ph.D.
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Abstract

Objective

To investigate the quantity of 2 stem cell types in the endometrial stroma of women undergoing in vitro fertilization and their association with steroid hormone signaling and implantation success after embryo transfer.

Design

Prospective cohort study.

Setting

Private hospital.

Patient(s)

A total of 109 patients undergoing in vitro fertilization.

Intervention(s)

Not applicable.

Main Outcome Measure(s)

Immunohistochemistry staining of endometrial biopsies taken during the midluteal phase using antibodies against NOTCH1 and CD117 was performed. The percentage of endometrial stromal cells positive for these markers was determined. The link of these stem cell percentages with the serum progesterone and estradiol levels and the endometrial expression of their respective receptors were assessed. After embryo transfer, the quantity of stained cells for each marker was also compared according to implantation outcome.

Result(s)

The percentage of NOTCH1+ stromal cells ranged from 0.003%–2.112% (median, 0.062%) and was significantly higher than that of CD117+ cells, which ranged from 0.000%–0.210% (median, 0.020%) (Z = −7.035). The percentage of NOTCH1+ stem cells showed no difference between the studied serum hormone level groups and no relationship with the expression of their receptors in the endometrium. In contrast, the number of CD117+ cells significantly differed between patients with high and low levels of serum progesterone (cutoff, 14.9 ng/mL) and estradiol (cutoff, 135.6 pg/mL). Furthermore, the quantity of CD117+ stem cells was positively correlated with the progesterone receptor (R = 0.277) and estradiol receptor (R= 0.318) expression levels in the endometrium. Although the quantity of NOTCH1+ cells did not differ between the 2 implantation groups, the median percentage of CD117+ cells was significantly higher in patients with successful implantation than in those with unsuccessful implantation (0.03% vs. 0.01%, respectively). The cutoff value for the percentage of CD117+ cells predicting successful implantation was 0.018% (area under the curve, 0.66; 95% confidence interval, 0.56–0.77; sensitivity, 63.1%; specificity, 61.4%).

Conclusion(s)

This study indicates that the quantity of certain stem cell types (CD117+), but not others (NOTCH1+), in the functional endometrium is associated with implantation success and sex hormone signaling during the midluteal phase. These findings highlight the role of CD117+ cells in preparing the endometrium for embryo implantation, and their quantity may be an indirect indicator of endometrial receptivity.

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人子宫内膜NOTCH1-和cd117阳性干细胞及其对成功着床的影响
目的探讨体外受精妇女子宫内膜基质中2种干细胞的数量及其与胚胎移植后类固醇激素信号和着床成功的关系。前瞻性队列研究。私立医院。病人:共109名接受体外受精的病人。干预措施不适用。主要观察指标(s)采用NOTCH1和CD117抗体对黄体中期子宫内膜活检进行免疫组化染色。测定这些标志物阳性的子宫内膜基质细胞的百分比。这些干细胞百分比与血清黄体酮和雌二醇水平以及各自受体的子宫内膜表达之间的联系进行了评估。结果(s) NOTCH1+基质细胞的比例为0.003% ~ 2.112%(中位数,0.062%),显著高于CD117+基质细胞的比例0.000% ~ 0.210%(中位数,0.020%)(Z = - 7.035)。NOTCH1+干细胞的百分比在研究的血清激素水平组之间没有差异,与其在子宫内膜中受体的表达无关。相比之下,血清黄体酮(截止值为14.9 ng/mL)和雌二醇(截止值为135.6 pg/mL)高、低水平患者的CD117+细胞数量有显著差异。CD117+干细胞数量与子宫内膜孕酮受体(R= 0.277)和雌二醇受体(R= 0.318)表达水平呈正相关。虽然NOTCH1+细胞的数量在两组之间没有差异,但CD117+细胞的中位数百分比在植入成功的患者中明显高于植入不成功的患者(分别为0.03%和0.01%)。预测成功植入的CD117+细胞百分比的截止值为0.018%(曲线下面积0.66;95%置信区间为0.56 ~ 0.77;敏感性,63.1%;结论:本研究提示功能子宫内膜中某些干细胞类型(CD117+)而非其他干细胞类型(NOTCH1+)的数量与着床成功和黄体中期性激素信号相关。这些发现强调了CD117+细胞在子宫内膜准备胚胎着床中的作用,它们的数量可能是子宫内膜容受性的间接指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
F&S science
F&S science Endocrinology, Diabetes and Metabolism, Obstetrics, Gynecology and Women's Health, Urology
CiteScore
2.00
自引率
0.00%
发文量
0
审稿时长
51 days
期刊最新文献
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