Awatif AlAnazi, Amer Nadeem, Khawar Siddiqui, Ali AlAhmari, Ibrahim Ghemlas, Abdullah AlJefri, Hawazen AlSaedi, Saadiya Khan, Mouhab Ayas
{"title":"Can the bone marrow harvest volume be reduced safely in hematopoietic stem cell transplantation with pediatric sibling donors?","authors":"Awatif AlAnazi, Amer Nadeem, Khawar Siddiqui, Ali AlAhmari, Ibrahim Ghemlas, Abdullah AlJefri, Hawazen AlSaedi, Saadiya Khan, Mouhab Ayas","doi":"10.5045/br.2023.2022167","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Reduced harvest volumes in pediatric donors appear to have the potential to reduce donor-associated risks while maintaining engraftment in recipients; however, the allowable harvest volume reduction remains undefined.</p><p><strong>Methods: </strong>We retrospectively analyzed the data pairs of 553 bone marrow (BM) harvests from pediatric (age at harvest <18 yr) sibling donors and clinical outcomes of 553 pediatric (age at infusion <14 yr) transplant-naïve recipients to assess the optimal BM harvest volume needed from pediatric donors to obtain the desired CD34+ cell count (≥3.0×10<sup>6</sup> cells per kg of recipient weight), and to study its impact on the clinical outcomes of transplantation in pediatric recipients.</p><p><strong>Results: </strong>The minimum desired CD34+ cell count of ≥3.0×10<sup>6</sup> per kg of recipient weight was achieved for 506 (95.3%) of donor-recipient pairs. The median CD34+ cell yield was 6.4×10<sup>6</sup> per kg of recipient weight (range, 1.2‒33.8×10<sup>6</sup>) in donors younger than 5 years old at harvest, 4.7×10<sup>6</sup> (range, 0.3‒28.5×10<sup>6</sup>) in donors aged 5‒10 years and 2.1×10<sup>6</sup> (range, 0.3‒11.3×10<sup>6</sup>) in donors older than 10 years (<i>P</i><0.001).</p><p><strong>Conclusion: </strong>The infused CD34+ cell dose (×10<sup>6</sup> cells/kg of recipient weight) had no impact on GRFS; however, a CD34+ cell dose of >7×10<sup>6</sup> cells/kg of recipient weight did not improve hematopoietic recovery.</p>","PeriodicalId":46224,"journal":{"name":"Blood Research","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/41/47/br-58-1-28.PMC10063592.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Blood Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5045/br.2023.2022167","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"HEMATOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Reduced harvest volumes in pediatric donors appear to have the potential to reduce donor-associated risks while maintaining engraftment in recipients; however, the allowable harvest volume reduction remains undefined.
Methods: We retrospectively analyzed the data pairs of 553 bone marrow (BM) harvests from pediatric (age at harvest <18 yr) sibling donors and clinical outcomes of 553 pediatric (age at infusion <14 yr) transplant-naïve recipients to assess the optimal BM harvest volume needed from pediatric donors to obtain the desired CD34+ cell count (≥3.0×106 cells per kg of recipient weight), and to study its impact on the clinical outcomes of transplantation in pediatric recipients.
Results: The minimum desired CD34+ cell count of ≥3.0×106 per kg of recipient weight was achieved for 506 (95.3%) of donor-recipient pairs. The median CD34+ cell yield was 6.4×106 per kg of recipient weight (range, 1.2‒33.8×106) in donors younger than 5 years old at harvest, 4.7×106 (range, 0.3‒28.5×106) in donors aged 5‒10 years and 2.1×106 (range, 0.3‒11.3×106) in donors older than 10 years (P<0.001).
Conclusion: The infused CD34+ cell dose (×106 cells/kg of recipient weight) had no impact on GRFS; however, a CD34+ cell dose of >7×106 cells/kg of recipient weight did not improve hematopoietic recovery.