A Preliminary Study on the Evaluation of Human Sperm Head Morphology with a Domestic Digital Holographic Image System.

IF 3.7 Q2 GENETICS & HEREDITY Phenomics (Cham, Switzerland) Pub Date : 2022-04-01 DOI:10.1007/s43657-022-00046-1

Hong Zhu, Yong Zhu, Can Sun, Feng Jiang

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引用次数: 2

Abstract

The head of sperm was imaged with domestic digital holographic microscopy (DHM), and then the quantitative three-dimensional size information of normal sperm and teratozoospermic sperm was compared and analyzed. DHM sperm imaging and repeated quantitative evaluation were used to determine the morphology of the sperm head in two patients with teratozoospermia and four volunteers with normal semen parameters. Sixty and 139 sperm of teratozoospermia patients and normal people were photographed by digital hologram, respectively. The differences in head height and width were compared and statistically analyzed. The sperm head height of the teratozoospermia group was 3.06 ± 1.66 μm, which was significantly lower than that of the normal sperm group (4.54 ± 1.60 μm, p < 0.01), but there was no significant difference in the head width between the two groups. Compared with the traditional two-dimensional optical microscope observation method, the DHM system can provide three-dimensional quantitative information for the sperm head and thus may help in the comprehensive clinical evaluation of the sperm head structure.

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用国产数字全息成像系统评价人类精子头部形态的初步研究。

采用国产数字全息显微镜(DHM)对精子头部进行成像，并对正常精子和畸形精子的定量三维尺寸信息进行比较分析。采用DHM精子成像和重复定量评价方法，对2例畸形精子症患者和4例精液参数正常的志愿者的精子头部形态进行了测定。对畸形精子症患者和正常人的精子分别进行了60个和139个的数字全息摄影。比较头高和头宽的差异，并进行统计学分析。畸形精子症组精子头高为3.06±1.66 μm，显著低于正常精子组(4.54±1.60 μm)

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Phenomics (Cham, Switzerland)

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