Neuroprotective Effect of Morinda citrifolia on Behavioural and Biochemical Deficits in PTZ-induced Kindled Mice.

Q3 Pharmacology, Toxicology and Pharmaceutics Infectious disorders drug targets Pub Date : 2023-06-05 DOI:10.2174/1871526523666230605160222
Moazzam Ali, Prabhat Singh, Lubhan Singh, Rupesh Kumar Pandey, Priyadarshini Soni, Akansha Singh
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Abstract

Introduction: Epilepsy is a group of chronic neurological disorders characterized by seizures. Kindling, a chronic epileptic mouse model, was used to explore the epileptogenic mechanism and seek new anti-epileptics. In kindling, sub-convulsive (chemical/ electrical) stimuli were delivered repeatedly and erratically, eventually causing massive convulsions. Moreover, Morinda citrifolia (Noni) extracts are used as a remedy in ayurvedic preparations for many ailments. Noni has recently been shown to protect mice from amyloid beta-induced memory loss.

Objective: This study was used to investigate the neuroprotective potential of Morinda citrifolia in mice over pentylenetetrazol (PTZ)-induced kindling seizure.

Methods: Kindling was provoked by subsequent (one-day-gap) injections of PTZ (subconvulsive; 35 mg/kg; s.c.) for 29 days in mice. Following PTZ injection, convulsive behaviours were noted for 30 minutes. Open-field-test (locomotor activity), forced swimming test (depressive behaviors), elevated plus-maze, and passive avoidance tests were employed to evaluate cognition. Brain homogenate was used to estimate oxidative stress (glutathione, superoxide-dismutase, lipid-peroxidation) and acetylcholinesterase activity.

Results: PTZ-provoked kindled mice displayed depressive behaviors, impaired locomotion, cognitive dysfunctions and various biochemical changes. However, treatment with Morinda citrifolia extract (500 and 1000 mg/kg, p.o) and valproic acid (200 mg/kg, p.o) before 60 min of each PTZ injection diminished kindling scores and restored behavioural, and biochemical changes.

Conclusion: Our findings suggest Morinda citrifolia offered neuroprotective effects against PTZinduced kindling seizures in mice, which were established by behavioural and biochemical paradigms.

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桑椹对ptz点燃小鼠行为和生化缺陷的神经保护作用。
癫痫是一组以癫痫发作为特征的慢性神经系统疾病。采用慢性癫痫小鼠模型Kindling,探讨其致痫机制,寻找新的抗癫痫药物。在点火过程中,亚惊厥(化学/电)刺激反复且不规律地传递,最终引起大面积惊厥。此外,桑葚(诺丽)提取物被用作阿育吠陀制剂中治疗许多疾病的药物。诺丽最近被证明可以保护老鼠免受淀粉样蛋白诱发的记忆丧失。目的:研究桑叶对戊四唑(PTZ)致小鼠点燃性癫痫的神经保护作用。方法:随后(间隔1天)注射PTZ(亚惊厥;35毫克/公斤;S.c .)在小鼠体内持续29天。注射PTZ后,惊厥行为持续30分钟。采用开放场地测试(运动活动)、强迫游泳测试(抑郁行为)、升高+迷宫和被动回避测试评估认知。脑匀浆用于评估氧化应激(谷胱甘肽、超氧化物歧化酶、脂质过氧化)和乙酰胆碱酯酶活性。结果:ptz点燃小鼠表现出抑郁行为、运动障碍、认知功能障碍和各种生化变化。然而,在每次PTZ注射前60分钟给予桑葚叶提取物(500和1000 mg/kg, p.o)和丙戊酸(200 mg/kg, p.o)治疗可降低点燃评分,恢复行为和生化变化。结论:从行为学和生物化学的角度看,桑叶对ptz致小鼠点燃性癫痫具有一定的神经保护作用。
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来源期刊
Infectious disorders drug targets
Infectious disorders drug targets Pharmacology, Toxicology and Pharmaceutics-Pharmacology
CiteScore
3.10
自引率
0.00%
发文量
123
期刊介绍: Infectious Disorders - Drug Targets aims to cover all the latest and outstanding developments on the medicinal chemistry, pharmacology, molecular biology, genomics and biochemistry of contemporary molecular targets involved in infectious disorders e.g. disease specific proteins, receptors, enzymes, genes. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics on drug targets involved in infectious disorders. As the discovery, identification, characterization and validation of novel human drug targets for anti-infective drug discovery continues to grow, this journal will be essential reading for all pharmaceutical scientists involved in drug discovery and development.
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