Characterization of γ-glutamyltransferase- and phytochelatin synthase-mediated catabolism of glutathione and glutathione S-conjugates in Arabidopsis thaliana.

IF 1.4 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Plant Biotechnology Pub Date : 2022-12-25 DOI:10.5511/plantbiotechnology.22.1003a

Ryota Inoue, Naoto Nakamura, Chie Matsumoto, Hisabumi Takase, Jiro Sekiya, Rafael Prieto

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引用次数: 1

Abstract

Glutathione (GSH, γ-L-glutamyl-L-cysteinyl-glycine) has been implicated in a multitude of cellular functions, such as protection of cells against oxidative stress, detoxification of xenobiotics via degradation of GSH S-conjugates, and disease resistance. Glutathione also serves as a precursor of phytochelatins, and thereby plays an essential role in heavy metal detoxification. The Arabidopsis genome encodes three functional γ-glutamyltransferase genes (AtGGT1, AtGGT2, AtGGT4) and two phytochelatin synthase genes (AtPCS1, AtPCS2). The function of plant GGT has not yet been clearly defined, although it is thought to be involved in GSH and GSH S-conjugate catabolism. On the other hand, besides its role in heavy metal detoxification, PCS has also been involved in GSH S-conjugate catabolism. Herein we describe the HPLC characterization of GSH and GSH S-conjugate catabolism in Arabidopsis mutants deficient in GSH biosynthesis (pad2-1/gsh1), atggt and atpcs1 T-DNA insertion mutants, atggt pad2-1, atggt atpcs1 double mutants, and the atggt1 atggt4 atpcs1 triple mutant. The results of our HPLC analysis confirm that AtGGT and AtPCS play important roles in two different pathways related with GSH and GSH S-conjugate (GS-bimane) catabolism in Arabidopsis.

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拟南芥γ-谷氨酰基转移酶和植物螯合素合成酶介导的谷胱甘肽和谷胱甘肽s偶联物的分解代谢特性

谷胱甘肽(γ- l-谷氨酰- l-半胱氨酸-甘氨酸)与多种细胞功能有关，如保护细胞免受氧化应激，通过降解谷胱甘肽s偶联物来解毒，以及抗病性。谷胱甘肽也是植物螯合素的前体，因此在重金属解毒中起重要作用。拟南芥基因组编码3个功能性γ-谷氨酰转移酶基因(AtGGT1、AtGGT2、AtGGT4)和2个植物螯合素合成酶基因(AtPCS1、AtPCS2)。植物GGT的功能尚未明确，尽管它被认为参与谷胱甘肽和谷胱甘肽s缀合物的分解代谢。另一方面，PCS除了具有重金属解毒作用外，还参与谷胱甘肽s缀合物的分解代谢。本文描述了GSH生物合成缺陷拟南芥突变体(pad2-1/gsh1)、atggt和atpcs1 T-DNA插入突变体、atggt pad2-1、atggt atpcs1双突变体和atggt1 atggt4 atpcs1三突变体中GSH和GSH s共轭分解代谢的HPLC表征。我们的HPLC分析结果证实，AtGGT和AtPCS在拟南芥GSH和GSH - s共轭物(gs -双烷)分解代谢的两个不同途径中发挥重要作用。

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来源期刊

Plant Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-PLANT SCIENCES

CiteScore

2.90

自引率

18.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Plant Biotechnology is an international, open-access, and online journal, published every three months by the Japanese Society for Plant Biotechnology. The journal, first published in 1984 as the predecessor journal, “Plant Tissue Culture Letters” and became its present form in 1997 when the society name was renamed to Japanese Society for Plant Cell and Molecular Biology, publishes findings in the areas from basic- to application research of plant biotechnology. The aim of Plant Biotechnology is to publish original and high-impact papers, in the most rapid turnaround time for reviewing, on the plant biotechnology including tissue culture, production of specialized metabolites, transgenic technology, and genome editing technology, and also on the related research fields including molecular biology, cell biology, genetics, plant breeding, plant physiology and biochemistry, metabolic engineering, synthetic biology, and bioinformatics.