Making plant methane formation visible-Insights from application of 13C-labeled dimethyl sulfoxide.

Abstract

Methane (CH₄) formation by vegetation has been studied intensively over the last 15 years. However, reported CH₄ emissions vary by several orders of magnitude, thus making global estimates difficult. Moreover, the mechanism(s) for CH₄ formation by plants is (are) largely unknown.Here, we introduce a new approach for making CH₄ formation by plants clearly visible. By application of ¹³C-labeled dimethyl sulfoxide (DMSO) onto the leaves of tobacco plants (Nicotiana tabacum) and Chinese silver grass (Miscanthus sinensis) the effect of light and dark conditions on CH₄ formation of this pathway was examined by monitoring stable carbon isotope ratios of headspace CH₄ (δ¹³C-CH₄ values).Both plant species showed increasing headspace δ¹³C-CH₄ values while exposed to light. Higher light intensities increased CH₄ formation rates in N. tabacum but decreased rates for M. sinensis. In the dark no formation of CH₄ could be detected for N. tabacum, while M. sinensis still produced ~50% of CH₄ compared to that during light exposure.Our findings suggest that CH₄ formation is clearly dependent on light conditions and plant species and thus indicate that DMSO is a potential precursor of vegetative CH₄. The novel isotope approach has great potential to investigate, at high temporal resolution, physiological, and environmental factors that control pathway-specific CH₄ emissions from plants.