EMP1 as a Potential Biomarker in Liver Fibrosis: A Bioinformatics Analysis.

IF 2 4区 医学 Q3 GASTROENTEROLOGY & HEPATOLOGY Gastroenterology Research and Practice Pub Date : 2023-01-01 DOI:10.1155/2023/2479192
Xuchen Chen, Xinliang Lv, Manman Han, Yexiao Hu, Wanqiong Zheng, Haibo Xue, Zhuokai Li, Kui Li, Wei Tan
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Abstract

Liver fibrosis is a wound-healing response to chronic injury, which may result in cirrhosis and liver failure. Studies have been carried on the mechanisms and pathogenesis of liver fibrosis. However, the potential cell-specific expressed marker genes involved in fibrotic processes remain unknown. In this study, we combined a publicly accessible single-cell transcriptome of human liver with microarray datasets to evaluate the cell-specific expression patterns of differentially expressed genes in the liver. We noticed that EMP1 (epithelial membrane protein 1) is significantly active not only in CCl4 (carbon tetrachloride)-treated mouse liver fibrosis but also in BDL (bile duct ligation)-induced liver fibrosis and even in human fibrotic liver tissues such as alcoholic hepatitis, NASH (nonalcoholic steatohepatitis), and advanced stage liver fibrosis. Furthermore, we demonstrated that EMP1 is a specific fibrotic gene expressed in HSCs (hepatic stellate cells) and endothelial cells using the Protein Atlas single-cell transcriptome RNA-sequencing clustering. Its expression was significantly elevated in fibrotic HSCs or CCl4 and NASH-induced fibroblasts. Previous research revealed that EMP1 plays a role in proliferation, migration, metastasis, and tumorigeneses in different cancers via a variety of mechanisms. Because HSC activation and proliferation are two important steps following liver injury, it would be interesting to investigate the role of EMP1 in these processes. All of this information suggested that EMP1 could be used as a novel fibrotic liver marker and a possible target in the future.

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EMP1作为肝纤维化的潜在生物标志物:生物信息学分析。
肝纤维化是对慢性损伤的一种伤口愈合反应,可导致肝硬化和肝功能衰竭。人们对肝纤维化的机制和发病机制进行了研究。然而,参与纤维化过程的潜在细胞特异性表达标记基因仍然未知。在这项研究中,我们将可公开获取的人类肝脏单细胞转录组与微阵列数据集相结合,以评估肝脏中差异表达基因的细胞特异性表达模式。我们注意到EMP1(上皮膜蛋白1)不仅在CCl4(四氯化碳)处理的小鼠肝纤维化中,而且在BDL(胆管结肠炎)诱导的肝纤维化中,甚至在人类纤维化肝组织中,如酒精性肝炎、NASH(非酒精性脂肪性肝炎)和晚期肝纤维化中,都具有显著的活性。此外,我们利用Protein Atlas单细胞转录组rna测序聚类,证明了EMP1是在hsc(肝星状细胞)和内皮细胞中表达的特异性纤维化基因。其在纤维化hsc、CCl4和nash诱导的成纤维细胞中的表达显著升高。先前的研究表明,EMP1通过多种机制在不同癌症的增殖、迁移、转移和肿瘤发生中发挥作用。由于HSC的激活和增殖是肝损伤后的两个重要步骤,因此研究EMP1在这些过程中的作用将是有趣的。所有这些信息表明,EMP1可以作为一种新的纤维化肝标志物和未来可能的靶点。
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来源期刊
Gastroenterology Research and Practice
Gastroenterology Research and Practice GASTROENTEROLOGY & HEPATOLOGY-
CiteScore
4.40
自引率
0.00%
发文量
91
审稿时长
1 months
期刊介绍: Gastroenterology Research and Practice is a peer-reviewed, Open Access journal which publishes original research articles, review articles and clinical studies based on all areas of gastroenterology, hepatology, pancreas and biliary, and related cancers. The journal welcomes submissions on the physiology, pathophysiology, etiology, diagnosis and therapy of gastrointestinal diseases. The aim of the journal is to provide cutting edge research related to the field of gastroenterology, as well as digestive diseases and disorders. Topics of interest include: Management of pancreatic diseases Third space endoscopy Endoscopic resection Therapeutic endoscopy Therapeutic endosonography.
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