Correlation of hepatic copper levels, rhodanine scores and histological diagnosis in archived canine liver samples.

IF 1.1 4区 农林科学 Q2 Veterinary Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2022-10-01
Ashish Gupta, Ahmad Al-Dissi
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Abstract

The liver is the main storage site for copper. Excess copper accumulation, however, is a risk factor for the development of chronic hepatitis in dogs. Mass spectrometry or rhodanine staining are frequently used methods to assess copper levels in the liver. The association was studied between analytic hepatic copper levels and rhodanine scores in archived canine formalin-fixed-paraffinembedded liver sections from 2014 to 2021 with various diagnoses. Thirty-six (N = 36) liver samples with analytic interpretation of toxic (n = 12), high normal (n = 17), and normal (n = 7) copper levels were selected for the study. Rhodanine staining for each of these samples was graded (scale: 1 to 5), and the association was determined between actual liver copper levels and rhodanine scores and histological diagnoses (chronic hepatitis or other diagnoses). The analytic copper level and rhodanine scores were significantly higher (P < 0.05) in samples designated as toxic compared to normal. There was a significant association between hepatic copper levels and rhodanine scores (P < 0.05). Rhodanine score, but not the actual liver copper levels were significantly (P < 0.05) associated with chronic hepatitis versus other diagnoses. Rhodanine scores of ≥ 1.89 were statistically significant predictors of chronic hepatitis. It was concluded from this study that actual liver copper levels are positively associated with rhodanine scores and rhodanine scores can be a useful predictor of chronic hepatitis.

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犬肝脏标本中肝铜水平、罗丹宁评分与组织学诊断的相关性。
肝脏是铜的主要储存场所。然而,过量的铜积累是狗患慢性肝炎的一个危险因素。质谱法或罗丹宁染色是常用的方法来评估铜在肝脏中的水平。研究2014年至2021年不同诊断的犬福尔马林固定石蜡包埋肝脏切片分析肝铜水平与罗丹宁评分之间的关系。36例肝脏样本(N = 36),铜含量分析解释为毒性(N = 12)、高正常(N = 17)和正常(N = 7)。每个样本的罗丹宁染色被分级(等级:1到5),并确定实际肝铜水平与罗丹宁评分和组织学诊断(慢性肝炎或其他诊断)之间的关系。毒性样品的分析铜水平和罗丹宁评分明显高于正常样品(P < 0.05)。肝铜水平与罗丹宁评分有显著相关性(P < 0.05)。与其他诊断相比,罗丹宁评分与慢性肝炎有显著相关性(P < 0.05),而实际肝铜水平与慢性肝炎无显著相关性(P < 0.05)。罗丹宁评分≥1.89是慢性肝炎有统计学意义的预测因子。本研究得出结论,实际肝铜水平与罗丹宁评分呈正相关,罗丹宁评分可作为慢性肝炎的有效预测指标。
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来源期刊
CiteScore
1.80
自引率
0.00%
发文量
58
审稿时长
>24 weeks
期刊介绍: The Canadian Journal of Veterinary Research, published by the Canadian Veterinary Medical Association, is Canada''s only veterinary research publication. This quarterly peer-reviewed online-only journal has earned a wide international readership through the publishing of high quality scientific papers in the field of veterinary medicine. The Journal publishes the results of original research in veterinary and comparative medicine.
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