Effect of in vitro gastrointestinal digestion on the chemical composition and antioxidant properties of Ginkgo biloba leaves decoction and commercial capsules.

IF 2.1 4区医学 Q3 PHARMACOLOGY & PHARMACY Acta Pharmaceutica Pub Date : 2022-12-01 DOI:10.2478/acph-2022-0033

Yubo Zhou, Yingxin Yang, Minyan Ma, Lingyun Xie, Aijuan Yan, Wen Cao

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Abstract

In this study Ginkgo biloba leaves (GBL) decoction and commercial capsules were digested using an in vitro model. Thirty-six active compounds were identified and quantified by HPLC-ESI-MS analysis based on the MS/MS patterns (precursor ions and product ions) and retention times, in comparison with reference standards. Most compounds in GBL showed a significant decrease during intestinal digestion, with an exception of vanillic acid and biflavonoids. Bioaccessibility values of chemical compositions varied between decoction and capsules samples. Also, significant reductions of total flavonoids and total phenolic content was observed after in vitro digestion. Both, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazo-line-6-sulfonic acid (ABTS) scavenging capacity decreased after gastric digestion, but increased during intestinal digestion. Nevertheless, different behaviour was observed in reducing antioxidant power (FRAP) assay. Compared to the pH of digestion, the influence of digestive enzymes on the chemical composition and antioxidant activity of GBL was relatively minor. Overall, these results may help provide a valid foundation for further investigations on bioactive compounds and the pharmacodynamics of GBL.

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体外胃肠消化对银杏叶煎剂及商业胶囊化学成分及抗氧化性能的影响。

以银杏叶(GBL)煎剂和银杏叶胶囊为研究对象，建立了银杏叶体外消化模型。采用HPLC-ESI-MS方法，根据质谱图(前体离子和产物离子)和保留时间对36种活性化合物进行鉴定和定量，并与对照品进行比较。除香草酸和生物黄酮外，GBL中大多数化合物在肠道消化过程中显著减少。化学成分的生物可及性值在汤剂和胶囊样品之间存在差异。体外消化后，总黄酮和总酚含量显著降低。2,2-二苯基-1-吡啶肼(DPPH)和2,2'-氮基-双(3-乙基苯并噻唑-线-6-磺酸(ABTS))清除能力在胃消化后均下降，在肠消化时增加。然而，在降低抗氧化能力(FRAP)试验中观察到不同的行为。与消化pH相比，消化酶对GBL化学成分和抗氧化活性的影响相对较小。这些结果为进一步研究GBL的生物活性化合物和药效学提供了有效的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Pharmaceutica PHARMACOLOGY & PHARMACY-

CiteScore

5.20

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： AP is an international, multidisciplinary journal devoted to pharmaceutical and allied sciences and contains articles predominantly on core biomedical and health subjects. The aim of AP is to increase the impact of pharmaceutical research in academia, industry and laboratories. With strong emphasis on quality and originality, AP publishes reports from the discovery of a drug up to clinical practice. Topics covered are: analytics, biochemistry, biopharmaceutics, biotechnology, cell biology, cell cultures, clinical pharmacy, drug design, drug delivery, drug disposition, drug stability, gene technology, medicine (including diagnostics and therapy), medicinal chemistry, metabolism, molecular modeling, pharmacology (clinical and animal), peptide and protein chemistry, pharmacognosy, pharmacoepidemiology, pharmacoeconomics, pharmacodynamics and pharmacokinetics, protein design, radiopharmaceuticals, and toxicology.