Amaryllidaceae alkaloids in skin cancer management: Photoprotective effect on human keratinocytes and anti-proliferative activity in melanoma cells.

IF 2 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Journal of applied biomedicine Pub Date : 2023-04-01 DOI:10.32725/jab.2023.004
Carol Castaneda, Karent Bravo, Natalie Cortes, Janeth Bedoya, Warley S de Borges, Jaume Bastida, Edison Osorio
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引用次数: 2

Abstract

Skin cancer has high rates of mortality and therapeutic failure. In this study, to develop a multi-agent strategy for skin cancer management, the selective cytotoxicity of several alkaloid fractions and pure alkaloids isolated from Amaryllidaceae species was evaluated in melanoma cells. In addition, UVB-stimulated keratinocytes (HaCaT) were exposed to seven alkaloid fractions characterized by GC-MS, and the production of intracellular reactive oxygen species (ROS) and IL-6, were measured to evaluate their photoprotection effects. The Eucharis caucana (bulb) alkaloid fraction (20 μg/ml) had a clear effect on the viability of melanoma cells, reducing it by 45.7% without affecting healthy keratinocytes. This alkaloid fraction and tazettine (both at 2.5 μg/ml) suppressed UVB-induced ROS production by 31.6% and 29.4%, respectively. The highest anti-inflammatory potential was shown by the Zephyranthes carinata (bulb) alkaloid fraction (10 μg/ml), which reduced IL-6 production by 90.8%. According to the chemometric analysis, lycoramine and tazettine had a photoprotective effect on the UVB-exposed HaCaT cells, attenuating the production of ROS and IL-6. These results suggest that Amaryllidaceae alkaloids have photoprotective and therapeutic potential in skin cancer management, especially at low concentrations.

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紫丁香科生物碱在皮肤癌管理中的作用:对人角质形成细胞的光保护作用和黑色素瘤细胞的抗增殖活性。
皮肤癌的死亡率和治疗失败率很高。在这项研究中,为了开发一种多药物治疗皮肤癌的策略,研究了从Amaryllidaceae物种中分离的几种生物碱组分和纯生物碱在黑色素瘤细胞中的选择性细胞毒性。此外,将uvb刺激的角质形成细胞(HaCaT)暴露于7种生物碱组分中,通过GC-MS表征,并测量细胞内活性氧(ROS)和IL-6的产生,以评估其光保护作用。白桦(球茎)生物碱组分(20 μg/ml)对黑色素瘤细胞的活性有明显的影响,在不影响健康角质形成细胞的情况下,使黑色素瘤细胞的活性降低45.7%。该生物碱组分和他泽汀(均为2.5 μg/ml)对uvb诱导的ROS产生的抑制作用分别为31.6%和29.4%。莪术生物碱部位(10 μg/ml)的抗炎活性最强,可使IL-6的生成降低90.8%。化学计量学分析表明,lycoramine和tazetinine对uvb暴露的HaCaT细胞具有光保护作用,可抑制ROS和IL-6的产生。这些结果表明,Amaryllidaceae生物碱具有光保护和治疗皮肤癌的潜力,特别是在低浓度下。
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来源期刊
Journal of applied biomedicine
Journal of applied biomedicine PHARMACOLOGY & PHARMACY-
CiteScore
2.40
自引率
7.70%
发文量
13
审稿时长
>12 weeks
期刊介绍: Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.
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