Topical Intranasal Insulin Enhances Healing of Nasal Mucosa: An Experimental Animal Study.

IF 2.5 3区 医学 Q1 OTORHINOLARYNGOLOGY American Journal of Rhinology & Allergy Pub Date : 2023-05-01 Epub Date: 2022-11-16 DOI:10.1177/19458924221139018
Çağrı Külekci, Serdar Özer, Selin Önen, Petek Korkusuz, Taner Yılmaz
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引用次数: 1

Abstract

Objective: Aim of this study was to evaluate the effect of topical intranasal insulin on healing of nasal mucosa in a rat model.

Methods: Forty-eight Wistar rats, weighing between 250 and 300 g and aged 10-12 weeks were used and randomized into two equal groups. 1.9 mm curette was introduced through the left nostril and 1.9 mm mucosa from the left nasal septum was curetted. Postoperatively, animals in the control group received 1 mL of physiologic saline, 3 times a day in a nasal irrigation fashion. Animals in the experimental group received 1 mL of 5 IU/mL regular insulin in saline solution. Subjects were sacrificed after 5, 10, and 15 days and macroscopic and histomorphometric evaluations were performed.

Results: There were no mucosal synechiae and septal perforation macroscopically. Histological examination revealed that the defect size reduction was 21% in the saline group versus 56% in the insulin group on the fifth day (p = 0.006). There was 62% defect reduction in the saline group versus 79% in the insulin group on the 10th day (p = 0.034). On the 15th day, only 67% of saline group animals had complete defect closure, whereas 100% of animals treated with insulin had complete closure (92% vs 100% mucosal defect reduction, p = 0.036). Both edema and inflammation were less in the insulin group on 15th day (p = 0.006; p = 0.023, respectively).

Conclusion: The results from this study support the safety and efficacy of topical insulin on wound healing in the literature. This study could guide further experimental studies that examine human sinonasal wound healing.

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局部鼻内胰岛素促进鼻粘膜愈合的实验动物研究。
目的:本研究旨在评价局部鼻内胰岛素对大鼠鼻黏膜愈合的影响。方法:48只Wistar大鼠,体重250至300g,年龄10-12周,随机分为两组。1.9 mm刮匙通过左鼻孔引入,1.9 刮除左侧鼻中隔的mm粘膜。术后,对照组动物接受1 mL生理盐水,以鼻腔冲洗的方式每天3次。实验组动物接受1 mL生理盐水溶液中的5IU/mL常规胰岛素。在5、10和15天后处死受试者,并进行宏观和组织形态测量评估。结果:肉眼未见黏膜粘连和间隔穿孔。组织学检查显示,第5天,生理盐水组的缺损尺寸缩小了21%,而胰岛素组的缺损面积缩小了56%(p = 0.006)。在第10天,生理盐水组的缺损减少了62%,而胰岛素组的缺损降低了79%(p = 0.034)。在第15天,只有67%的生理盐水组动物完全闭合了缺损,而用胰岛素治疗的动物100%完全闭合(92%对100%粘膜缺损减少,p = 0.036)。胰岛素组在第15天水肿和炎症均减少(p = 0.006;p = 结论:本研究结果支持文献中外用胰岛素对伤口愈合的安全性和有效性。这项研究可以指导进一步的实验研究,以检查人类鼻腔伤口愈合。
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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
82
审稿时长
4-8 weeks
期刊介绍: The American Journal of Rhinology & Allergy is a peer-reviewed, scientific publication committed to expanding knowledge and publishing the best clinical and basic research within the fields of Rhinology & Allergy. Its focus is to publish information which contributes to improved quality of care for patients with nasal and sinus disorders. Its primary readership consists of otolaryngologists, allergists, and plastic surgeons. Published material includes peer-reviewed original research, clinical trials, and review articles.
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