Identification and characterization of differentially expressed circRNA in 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced cleft palate.

IF 2.7 4区医学 Q3 TOXICOLOGY Human & Experimental Toxicology Pub Date : 2023-01-01 DOI:10.1177/09603271231183359

Liyun Gao, Jingwen Tan, Chunhua Han, Junfei Fan, Jiayin He, Ting Luo, Shiqun Yu, Xiangxin Che, Lin Zhang, Xin Wang

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Abstract

Various circular RNAs (circRNAs) are novel class of non-coding RNAs, which are pervasively transcribed in the genome. CircRNAs play important roles in human, animals and plants. Up to now, there was no report regarding circRNAs of cleft palate by 2,3,7,8-tetrachlorodibenzo-pdioxin (TCDD) induce. The present study screened identification and characterization of differential expressed-circRNAs in TCDD-induced cleft palate. 6903 circRNAs candidates came from cleft palates. Among them, 3525 circRNAs are up-regulation, and 3378 circRNAs are down-regulation by TCDD induce. The cluster and GO analysis found that circRNAs involved in biological process, cellular component, and molecular function. Through the analysis of KEGG Pathway, circRNAs made functions via classical signaling pathway in cleft palate, such as TGF-beta signaling pathway, BMP signal pathway, MAPK signaling pathway. In addition, we found down-regulated circRNA224, circRNA3302 and up-regulated circRNA5021 targeted tgfbr3, but up-regulated circRNA4451 targeted tgfbr2. circRNA4451 may make functions through TGF-beta signaling pathway. These results suggested that many different circRNAs may make important role in TCDD-induced cleft palate, which provided a theoretical basis for further research.

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2,3,7,8-四氯二苯并-对二恶英所致腭裂中circRNA差异表达的鉴定与表征

各种环状rna (circRNAs)是一类新型的非编码rna，广泛存在于基因组中。circrna在人类、动物和植物中发挥着重要作用。到目前为止，还没有关于2,3,7,8-四氯二苯并二恶英(TCDD)诱发腭裂的环状rna的报道。本研究筛选了tcdd诱导腭裂中差异表达的环状rna的鉴定和表征。6903个环状rna候选基因来自腭裂。其中，3525个circrna被TCDD诱导上调，3378个circrna被TCDD诱导下调。聚类和氧化石墨烯分析发现，环状rna参与生物过程、细胞成分和分子功能。通过对KEGG通路的分析，circrna在腭裂中通过经典的信号通路发挥作用，如tgf - β信号通路、BMP信号通路、MAPK信号通路。此外，我们发现下调的circRNA224、circRNA3302和上调的circRNA5021靶向tgfbr3，而上调的circRNA4451靶向tgfbr2。circRNA4451可能通过tgf - β信号通路发挥作用。这些结果提示，许多不同的circrna可能在tcdd诱导的腭裂中发挥重要作用，为进一步的研究提供了理论基础。

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来源期刊

Human & Experimental Toxicology 医学-毒理学

CiteScore

5.70

自引率

3.60%

发文量

128

审稿时长

2.3 months

期刊介绍： Human and Experimental Toxicology (HET), an international peer reviewed journal, is dedicated to publishing preclinical and clinical original research papers and in-depth reviews that comprehensively cover studies of functional, biochemical and structural disorders in toxicology. The principal aim of the HET is to publish timely high impact hypothesis driven scholarly work with an international scope. The journal publishes on: Structural, functional, biochemical, and molecular effects of toxic agents; Studies that address mechanisms/modes of toxicity; Safety evaluation of novel chemical, biotechnologically-derived products, and nanomaterials for human health assessment including statistical and mechanism-based approaches; Novel methods or approaches to research on animal and human tissues (medical and veterinary patients) investigating functional, biochemical and structural disorder; in vitro techniques, particularly those supporting alternative methods