[An improved method about culture and identification of primary rat bone marrow derived mesenchymal stem cells].

Q4 Medicine Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology Pub Date : 2022-11-01 DOI:10.12047/j.cjap.6285.2022.134

Hua-Gen Ma, Hai-Qin Liu, Yuan-Yu Tang

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引用次数: 0

Abstract

目的: 改良原代大鼠骨髓间充质干细胞(BMSCs)的分离培养方法。方法: 基于“干细胞生态位”原理,采用全骨髓贴壁法结合骨片消化法分离培养原代大鼠BMSCs。通过细胞形态学观察、细胞表面标志物CD分子检测以及定向诱导分化实验对目的细胞进行鉴定。结果: 所培养的细胞形态为细长梭形,其融合后呈典型的“涡旋状”紧密排列,且细胞增殖迅速,原代培养5～6 d即可达到融合状态,按1∶3比例连续传代到第8代时,细胞形态仍基本保持不变;第4代细胞的间充质干细胞表面标志物CD90、CD29、CD73以及造血干细胞表面标志物CD45、CD34、CD11b/c的阳性表达率分别为89.0％、98.3％、64.2％、3.5％、5.6％、 24.7％;定向诱导分化实验表明目的细胞具有良好的成脂、成骨分化潜能。结论: 本研究基于“干细胞生态位”原理,成功建立了一种高效稳定的原代大鼠BMSCs分离培养方法。.

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