[Effects of PBMC mitochondrial autophagy on exercise energy consumption and mitochondrial respiratory chain complex enzyme activity in high altitude migrants].

Q4 Medicine Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology Pub Date : 2022-11-01 DOI:10.12047/j.cjap.6345.2022.119

Hai-Jun Kong, Feng-Hua Wang, Xin-Long Li, Sheng-Qian Gan, Xiao'an Chen

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引用次数: 0

Abstract

目的: 探究高原移居中青年男性走、跑状态运动能耗及外周血单个核细胞(PBMC)线粒体自噬标志物变化特征。方法: 招募152名受试者,根据移居高原年限分为高原移居Ⅰ组(HM1)、高原移居Ⅱ组(HM2)、高原移居Ⅲ组(HM3)、世居高原组(Con)。检测受试者3.0 km/h 、4.5 km/h、6.0 km/h及7.5 km/h条件下运动能耗;ELISA检测受试者PBMC线粒体NADH-Q氧化还原酶、琥珀酸-Q氧化还原酶、UQ-细胞色素C氧化还原酶、细胞色素C氧化酶水平;RT-PCR和Western blot检测受试者PBMC线粒体Parkin、PINK3、LC3A、LC3B mRNA及蛋白表达水平。结果: 与Con组比较,HM1、HM2组3 km/h步行能耗显著上升(P＜ 0.05);与HM1组比较,HM2组显著降低(P＜0.05) 。与Con组比较,HM1组4.5 km/h、6 km/h及7.5 km/h走、跑能耗均显著上升(P＜0.05) 。与Con组比较,HM1、HM2和HM3组复合物Ⅰ、复合物Ⅱ及复合物Ⅲ水平均显著降低(P＜0.05) ;与HM1组和HM2组比较,HM3组显著上升(P＜0.05) ;与HM1组比较,HM2组显著上升(P＜0.05) 。与Con组比较,HM1、HM2组复合物Ⅳ水平显著降低(P＜0.05) ;与HM1组和HM2组比较,HM3组显著上升(P＜0.05) 。与Con组比较,HM1、HM2和HM3组Parkin mRNA表达显著上升(P＜0.05) ,HM1、HM2组高于HM3组(P＜0.05) ;与Con组比较,HM1、HM2和HM3组PINK3 mRNA表达显著降低(P＜0.05) ,HM1组显著低于HM2、HM3组(P＜0.05) ;与Con组比较,HM1、HM2和HM3组LC3A mRNA表达显著降低(P＜0.05) ,HM1组和HM2组显著低于HM3组,HM1组显著低于HM2组(P＜0.05) ;与Con组比较,HM1、HM2和HM3组LC3B mRNA和LC3B蛋白表达显著上升(P＜0.05) ,HM1组和HM2组显著高于HM3组,HM1组显著低于HM2组(P＜0.05) 。与Con组比较,HM1、HM2组Parkin蛋白表达显著上升(P＜0.05) ,HM1组、HM2组显著高于HM3组(P＜0.05) ;与Con组比较,HM1、HM2组PINK3和LC3A蛋白表达显著降低(P＜0.05) ,HM1组、HM2组显著低于HM3组(P＜0.05) 。结论: 高原移居者随高原移居时间延长,PBMC线粒体自噬水平逐步下降,PBMC线粒体呼吸酶活性逐渐增殖;高原定量负荷运动能量消耗逐渐降低。.

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