Analyzing the Association of Visceral Adipose Tissue Growth Differentiation Factor-15 and MicroRNA in Type 2 Diabetes Mellitus.

IF 4.7 Q1 ENDOCRINOLOGY & METABOLISM Journal of Obesity & Metabolic Syndrome Pub Date : 2023-03-30 Epub Date: 2023-03-15 DOI:10.7570/jomes22010

Dipayan Roy, Purvi Purohit, Manoj Khokhar, Anupama Modi, Ravindra Kumar Gayaprasad Shukla, Ramkaran Chaudhary, Shrimanjunath Sankanagoudar, Praveen Sharma

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Abstract

Background: Growth differentiation factor-15 (GDF-15) is involved in insulin resistance and diabetes. In this study, we determine the associations of GDF-15 with miR-181b-5p, miR-330-3p, mothers against decapentaplegic homolog 7 (SMAD7), and insulin resistance in visceral adipose tissue (VAT) and peripheral blood mononuclear cells (PBMCs) in type 2 diabetes mellitus (T2DM) patients.

Methods: Sixty patients, equally divided into those with T2DM and non-diabetic controls, were recruited for gene expression analysis. Protein-protein interaction (STRING), target prediction (miRNet), and functional enrichment were conducted accordingly.

Results: Our study showed that VAT and PBMCs had similar expression profiles, where GDF-15 and miR-181b-5p were upregulated, whereas SMAD7 and miR-330-3p were downregulated. Serum GDF-15 could differentiate between T2DM and non-diabetic patients (P<0.001). Target prediction revealed a microRNA (miRNA)-messenger RNA regulatory network, transcription factors, and functional enrichment for the miRNA that suggested involvement in T2DM pathogenesis.

Conclusion: VAT GDF-15 is associated with insulin resistance and is possibly regulated by miR-181b-5p, miR-330-3p, and SMAD7 in T2DM.

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分析 2 型糖尿病患者内脏脂肪组织生长分化因子-15 与 MicroRNA 的关系

背景：生长分化因子-15（GDF-15）与胰岛素抵抗和糖尿病有关。在这项研究中，我们确定了 GDF-15 与 miR-181b-5p、miR-330-3p、母亲抗截瘫同源物 7（SMAD7）以及 2 型糖尿病（T2DM）患者内脏脂肪组织（VAT）和外周血单核细胞（PBMCs）中胰岛素抵抗的关系：招募 60 名患者进行基因表达分析，这些患者平均分为 T2DM 患者和非糖尿病对照组。结果：我们的研究显示，VAT 和 PAT 的基因表达与 T2DM 患者的基因表达存在显著差异：结果：我们的研究表明，VAT 和 PBMCs 有相似的表达谱，其中 GDF-15 和 miR-181b-5p 上调，而 SMAD7 和 miR-330-3p 下调。血清 GDF-15 可以区分 T2DM 患者和非糖尿病患者（结论：VAT GDF-15 与降糖药物有关：VAT GDF-15 与 T2DM 患者的胰岛素抵抗有关，并可能受 miR-181b-5p、miR-330-3p 和 SMAD7 的调控。

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来源期刊

Journal of Obesity & Metabolic Syndrome ENDOCRINOLOGY & METABOLISM-

CiteScore

8.30

自引率

9.60%

发文量

审稿时长

19 weeks

期刊介绍： The journal was launched in 1992 and diverse studies on obesity have been published under the title of Journal of Korean Society for the Study of Obesity until 2004. Since 2017, volume 26, the title is now the Journal of Obesity & Metabolic Syndrome (pISSN 2508-6235, eISSN 2508-7576). The journal is published quarterly on March 30th, June 30th, September 30th and December 30th. The official title of the journal is now "Journal of Obesity & Metabolic Syndrome" and the abbreviated title is "J Obes Metab Syndr". Index words from medical subject headings (MeSH) list of Index Medicus are included in each article to facilitate article search. Some or all of the articles of this journal are included in the index of PubMed, PubMed Central, Scopus, Embase, DOAJ, Ebsco, KCI, KoreaMed, KoMCI, Science Central, Crossref Metadata Search, Google Scholar, and Emerging Sources Citation Index (ESCI).