Molecular Characterization of Extended Spectrum β-Lactamase Producing Escherichia coli and Klebsiella pneumoniae Isolates and Their Antibiotic Resistance Profile in Health Care-Associated Urinary Tract Infections in North India.

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Journal of Laboratory Physicians Pub Date : 2023-06-01 DOI:10.1055/s-0042-1757416
Sheetal Verma, Raj Kumar Kalyan, Prashant Gupta, Mohd Danish Khan, Vimala Venkatesh
{"title":"Molecular Characterization of Extended Spectrum β-Lactamase Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolates and Their Antibiotic Resistance Profile in Health Care-Associated Urinary Tract Infections in North India.","authors":"Sheetal Verma,&nbsp;Raj Kumar Kalyan,&nbsp;Prashant Gupta,&nbsp;Mohd Danish Khan,&nbsp;Vimala Venkatesh","doi":"10.1055/s-0042-1757416","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background</b>  Healthcare-associated urinary tract infections (HAUTIs) caused by gram-negative pathogens have emerged as a global concern. So far, little is known about the epidemiology of extended-spectrum β lactamase (ESBL)-producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> in HAUTIs in India. The study was carried to determine the antibiotic resistance pattern and ESBL-producing genes in <i>E. coli</i> and <i>K. pneumoniae</i> strains isolated from HAUTIs in a tertiary institute in North India. <b>Methods</b>  A total of 200 consecutive, nonduplicate clinical isolates of <i>E. coli</i> and 140 isolates of <i>K. pneumoniae</i> from hospitalized patients with UTI were collected during a period of 1 year. Strains were studied for the presence of ESBL genes (blaCTX-M1, blaCTX-M2, blaCTX-M9, blaCTX-M15, blaSHV, blaTEM, blaOXA-1, blaVEB, blaPER-2, and blaGES) by multiplex polymerase chain reaction using gene-specific primers. <b>Results</b>  ESBL was detected in 82.5% (165 out of 200) isolates of <i>E. coli</i> and 74.3% (104 out of 140) isolates of <i>K. pneumoniae</i> by phenotypic confirmatory testing. From 269 phenotypically positive ESBL isolates, blaTEM (49.4%) was the most common genotype followed by blaCTX-M1 (31.97%), blaOXA-1 (30.1%), and blaSHV(11.9%) either alone or in combination. In the present study, blaCTX-M-15 (84.89%) was the most common blaCTX-M1-type ESBL. In total, 2.6 and 5.2% of the isolates were positive for <i>PER-2</i> and <i>VEB</i> genes, respectively. <b>Conclusion</b>  To the best of our knowledge, this is the first study on ESBL resistance patterns and ESBL-producing genes in HAUTIs in North India. Our study reports high occurrence with ESBL types CTX-M-1, CTX-M-15, TEM, and SHV. Minor ESBL variants OXA-1, VEB-type, and PER-2-type β-lactamase are also emerging in HAUTIs infections in North India.</p>","PeriodicalId":16149,"journal":{"name":"Journal of Laboratory Physicians","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/18/ac/10-1055-s-0042-1757416.PMC10264127.pdf","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Laboratory Physicians","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1055/s-0042-1757416","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
引用次数: 2

Abstract

Background  Healthcare-associated urinary tract infections (HAUTIs) caused by gram-negative pathogens have emerged as a global concern. So far, little is known about the epidemiology of extended-spectrum β lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae in HAUTIs in India. The study was carried to determine the antibiotic resistance pattern and ESBL-producing genes in E. coli and K. pneumoniae strains isolated from HAUTIs in a tertiary institute in North India. Methods  A total of 200 consecutive, nonduplicate clinical isolates of E. coli and 140 isolates of K. pneumoniae from hospitalized patients with UTI were collected during a period of 1 year. Strains were studied for the presence of ESBL genes (blaCTX-M1, blaCTX-M2, blaCTX-M9, blaCTX-M15, blaSHV, blaTEM, blaOXA-1, blaVEB, blaPER-2, and blaGES) by multiplex polymerase chain reaction using gene-specific primers. Results  ESBL was detected in 82.5% (165 out of 200) isolates of E. coli and 74.3% (104 out of 140) isolates of K. pneumoniae by phenotypic confirmatory testing. From 269 phenotypically positive ESBL isolates, blaTEM (49.4%) was the most common genotype followed by blaCTX-M1 (31.97%), blaOXA-1 (30.1%), and blaSHV(11.9%) either alone or in combination. In the present study, blaCTX-M-15 (84.89%) was the most common blaCTX-M1-type ESBL. In total, 2.6 and 5.2% of the isolates were positive for PER-2 and VEB genes, respectively. Conclusion  To the best of our knowledge, this is the first study on ESBL resistance patterns and ESBL-producing genes in HAUTIs in North India. Our study reports high occurrence with ESBL types CTX-M-1, CTX-M-15, TEM, and SHV. Minor ESBL variants OXA-1, VEB-type, and PER-2-type β-lactamase are also emerging in HAUTIs infections in North India.

Abstract Image

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
北印度卫生保健相关尿路感染中产生β-内酰胺酶的大肠杆菌和肺炎克雷伯菌的广谱分子特征及其抗生素耐药性
由革兰氏阴性病原体引起的卫生保健相关性尿路感染(HAUTIs)已成为全球关注的问题。迄今为止,对印度HAUTIs中产生广谱β内酰胺酶(ESBL)的大肠埃希菌和肺炎克雷伯菌的流行病学知之甚少。该研究是为了确定从印度北部一所高等院校的重症感染中分离的大肠杆菌和肺炎克雷伯菌菌株的抗生素耐药模式和产生esbl的基因。方法收集1年住院尿路感染患者连续分离的200株非重复临床分离的大肠杆菌和140株肺炎克雷伯菌。采用基因特异性引物进行多重聚合酶链反应,检测菌株是否存在ESBL基因(blaCTX-M1、blaCTX-M2、blaCTX-M9、blaCTX-M15、blaSHV、blaTEM、blaxa -1、blaVEB、blaPER-2和blaGES)。结果200株大肠杆菌中165株(82.5%)和140株肺炎克雷伯菌中104株(74.3%)经表型确证检测检出ESBL。在269株表型阳性的ESBL分离株中,最常见的基因型是blaTEM(49.4%),其次是blaCTX-M1(31.97%)、blaOXA-1(30.1%)和blaSHV(11.9%)。在本研究中,blaCTX-M-15(84.89%)是最常见的blactx - m1型ESBL。PER-2和VEB基因阳性率分别为2.6%和5.2%。据我们所知,这是对印度北部HAUTIs中ESBL抗性模式和产生ESBL基因的首次研究。我们的研究报告了ESBL型CTX-M-1、CTX-M-15、TEM和SHV的高发生率。轻微的ESBL变异OXA-1、veb型和per -2型β-内酰胺酶也出现在印度北部的HAUTIs感染中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of Laboratory Physicians
Journal of Laboratory Physicians MEDICINE, GENERAL & INTERNAL-
自引率
0.00%
发文量
99
审稿时长
31 weeks
期刊最新文献
Experience of rigorous practice of antimicrobial stewardship program and its impact on antibiotic consumption A new era for Journal of Laboratory Physicians Sensitivity and specificity of newly generated monoclonal antibodies to detect novel antigens of Mycobacterium tuberculosis for the diagnosis of all forms of tuberculosis Martin’s formula is best to calculate low-density lipoprotein cholesterol Performance of modified colistin broth disc elution vis-a-vis broth microdilution method for susceptibility testing of Enterobacterales
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1