IncRNA EPB41L4A-AS1 Mitigates the Proliferation of Non-Small-Cell Lung Cancer Cells through the miR-105-5p/GIMAP6 Axis.

IF 1.5 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Critical Reviews in Eukaryotic Gene Expression Pub Date : 2023-01-01 DOI:10.1615/CritRevEukaryotGeneExpr.2022044323
Bingwei Dong, Fenjuan Zhang, Weibo Zhang, Yingfang Gao
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引用次数: 1

Abstract

Non-small-cell lung cancer (NSCLC) is the major subtype of lung cancer, with a series of long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and proteins involved in its pathogenesis. This study sought to investigate the functionality of lncRNA EPB41L4A antisense RNA 1 (lncRNA EPB41L4A-AS1) in the proliferation of NSCLC cells and provide a novel theoretical reference for NSCLC treatment. Levels of lncRNA EPB41L4A-AS1, miR-105-5p, and GTPase, IMAP family member 6 (GIMAP6) in tissues and cells were measured by RT-qPCR and the correlation between lncRNA EPB41L4A-AS1 and clinicopathological characteristics was analyzed. Cell proliferation was evaluated by cell counting kit-8 and colony formation assays. The subcellular localization of lncRNA EPB41L4A-AS1 was analyzed by the subcellular fractionation assay and the binding of miR-105-5p to lncRNA EPB41L4A-AS1 or GIMAP6 was analyzed by dual-luciferase and RNA pull-down assays. Functional rescue experiments were performed to analyze the role of miR-105-5p/GIMAP6 in NSCLC cell proliferation. lncRNA EPB41L4A-AS1 and GIMAP6 were downregulated while miR-105-5p was upregulated in NSCLC tissues and cells. lncRNA EPB41L4A-AS1 was correlated with tumor size and clinical staging and its overexpression reduced NSCLC cell proliferation. lncRNA EPB41L4A-AS1 was negatively correlated with miR-105-5p and positively correlated with GIMAP6 in NSCLC tissues, and lncRNA EPB41L4A-AS1 sponged miR-105-5p to promote GIMAP6 transcription in NSCLC cells. Overexpression of miR-105-5p or knockdown of GIMAP6 reversed the inhibition of lncRNA EPB41L4A-AS1 overexpression on NSCLC cell proliferation. lncRNA EPB41L4A-AS1 was downregulated in NSCLC and mitigated NSCLC cell proliferation through the miR-105-5p/GI-MAP6 axis.

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IncRNA EPB41L4A-AS1通过miR-105-5p/GIMAP6轴减缓非小细胞肺癌细胞的增殖
非小细胞肺癌(Non-small-cell lung cancer, NSCLC)是肺癌的主要亚型,其发病机制涉及一系列长链非编码rna (lncRNAs)、microRNAs (miRNAs)和蛋白。本研究旨在探讨lncRNA EPB41L4A反义RNA 1 (lncRNA EPB41L4A- as1)在非小细胞肺癌细胞增殖中的功能,为非小细胞肺癌治疗提供新的理论参考。RT-qPCR检测组织和细胞中lncRNA EPB41L4A-AS1、miR-105-5p、GTPase、IMAP家族成员6 (GIMAP6)水平,分析lncRNA EPB41L4A-AS1与临床病理特征的相关性。通过细胞计数试剂盒-8和菌落形成试验评估细胞增殖。通过亚细胞分离法分析lncRNA EPB41L4A-AS1的亚细胞定位,通过双荧光素酶和RNA下拉法分析miR-105-5p与lncRNA EPB41L4A-AS1或GIMAP6的结合。通过功能挽救实验分析miR-105-5p/GIMAP6在NSCLC细胞增殖中的作用。在NSCLC组织和细胞中,lncRNA EPB41L4A-AS1和GIMAP6下调,miR-105-5p上调。lncRNA EPB41L4A-AS1与肿瘤大小和临床分期相关,其过表达可降低NSCLC细胞增殖。在非小细胞肺癌组织中,lncRNA EPB41L4A-AS1与miR-105-5p呈负相关,与GIMAP6呈正相关,并且lncRNA EPB41L4A-AS1对miR-105-5p进行海泡,促进GIMAP6在非小细胞肺癌细胞中的转录。过表达miR-105-5p或敲低GIMAP6可逆转lncRNA EPB41L4A-AS1过表达对NSCLC细胞增殖的抑制作用。lncRNA EPB41L4A-AS1在NSCLC中下调,并通过miR-105-5p/GI-MAP6轴减缓NSCLC细胞增殖。
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来源期刊
Critical Reviews in Eukaryotic Gene Expression
Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物
CiteScore
2.70
自引率
0.00%
发文量
67
审稿时长
1 months
期刊介绍: Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.
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