Analysis of pCl107 a large plasmid carried by an ST25 Acinetobacter baumannii strain reveals a complex evolutionary history and links to multiple antibiotic resistance and metabolic pathways.

Rayane Rafei, Jonathan Koong, Marwan Osman, Ahmad Al Atrouni, Monzer Hamze, Mehrad Hamidian
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引用次数: 3

Abstract

Acinetobacter baumannii has successfully spread during the last decades as one of the main critically important pathogens. However, many aspects including plasmids, are still under-investigated. Here, we report the complete sequence of an Acinetobacter baumannii strain, belonging to the ST25IP (Institut Pasteur) sequence type recovered in 2012 in Lebanon, using a combination of Illumina MiSeq and Oxford Nanopore sequencing and a hybrid assembly approach. This strain (Cl107) carries a 198 kb plasmid called pCl107 that encodes the MPFI conjugative transfer system. The plasmid carries the aacA1, aacC2, sul2, strAB, and tetA(B) antibiotic resistance genes. pCl107 region encompassing the sul2, strAB, tetA(B) is closely related to AbGRI1 chromosomal resistance islands, which are widespread in A. baumannii strains belonging to Global Clone 2. The resistance region found in pCl107 is one of the missing links in the evolutionary history of the AbGRI1 islands. pCl107 also contains a BREX Type 1 region and represents one of the two main evolution patterns observed in BREX clusters found in plasmids related to pCl107. pCl107 also harbours a ptx phosphonate metabolism module, which plays an ancestral structure compared to other large plasmids in ST25 strains. While the uric acid metabolic module found in pCl107 is incomplete, we identified possible ancestors from plasmids and chromosomes of Acinetobacter spp. Our analyses indicate a complex evolutionary history of plasmids related to pCl107 with many links to multiple antibiotic resistance and metabolic pathways.

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对鲍曼不动杆菌ST25携带的大质粒pCl107的分析揭示了其复杂的进化历史,并与多种抗生素耐药和代谢途径有关。
鲍曼不动杆菌在过去的几十年里作为一种重要的病原体成功地传播开来。然而,包括质粒在内的许多方面仍有待研究。在这里,我们报告了2012年在黎巴嫩恢复的属于ST25IP (Institut Pasteur)序列类型的鲍曼不动杆菌菌株的完整序列,使用Illumina MiSeq和Oxford Nanopore测序和混合组装方法的组合。该菌株(Cl107)携带一个198 kb的质粒pCl107,编码MPFI共轭转移系统。质粒携带aacA1、aacC2、sul2、strAB和tetA(B)抗生素耐药基因。包含sul2, strAB, tetA(B)的pCl107区域与AbGRI1染色体耐药岛密切相关,AbGRI1耐药岛在鲍曼鲍曼杆菌属全球克隆2菌株中广泛存在。在pCl107中发现的抗性区域是AbGRI1岛进化史上缺失的环节之一。pCl107还包含一个BREX 1型区域,代表了在pCl107相关质粒中发现的BREX簇中观察到的两种主要进化模式之一。pCl107还含有ptx膦酸盐代谢模块,与ST25菌株中的其他大质粒相比,pCl107具有祖先结构。虽然在pCl107中发现的尿酸代谢模块不完整,但我们从不动杆菌的质粒和染色体中发现了可能的祖先。我们的分析表明,与pCl107相关的质粒具有复杂的进化历史,与多种抗生素耐药性和代谢途径有许多联系。
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3.30
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审稿时长
15 weeks
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