Variable Virulence Genes in Clinical Isolates of Burkholderia pseudomallei : Impact on Disease Severity and Outcome in Melioidosis.

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Journal of Laboratory Physicians Pub Date : 2023-06-01 DOI:10.1055/s-0042-1760667
Sruthi Raj, Sujatha Sistla, Deepthy Melepurakkal Sadanandan, Sreeram Chandra Murthy Peela
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引用次数: 2

Abstract

Objectives  To isolate Burkholderia pseudomallei from clinical specimens and study the association of virulence genes with clinical manifestations and outcome in patients with melioidosis. Materials and MethodsBurkholderia pseudomallei isolates obtained from melioidosis cases diagnosed during 2018 to 2021 were identified using VITEK 2 system and confirmed by polymerase chain reaction (PCR) targeting a Type III secretion system gene cluster. Multiplex PCR was performed to detect the genotypes of lipopolysaccharide (LPS) namely A, B, and B2, and singleplex PCR was performed to detect the presence of the Burkholderia intracellular motility gene ( BimA ) and filamentous hemagglutinin gene ( fhaB3 ). Statistical Analysis  Chi-square/Fisher's exact tests were performed to study the association between various clinical manifestations and outcome and different virulence genes. The results were expressed as unadjusted odds ratios with 95% confidence intervals. Results  Sixty-seven isolates were available for characterization. BimA Bm and BimA Bp were observed among 82 and 18% of the isolates, respectively. Both sepsis and mortality were significantly associated with BimA Bm . Majority of the isolates had fhaB3 (97%). Most of the isolates showed the presence of LPS A gene (65.7%) followed by LPS B gene (6%), while LPS B2 was not detected. Nineteen isolates could not be assigned to any LPS genotypes. Conclusion  Among the virulence genes studied, only BimA Bm was significantly associated with sepsis and mortality. More than a quarter (28.3%) of the isolates could not be assigned to any LPS genotypes, hinting at a greater genetic diversity in our isolates.

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假马利氏伯克氏菌临床分离株的可变毒力基因:对类鼻疽疾病严重程度和结局的影响。
目的从临床标本中分离假马利氏伯克氏菌,研究其毒力基因与类鼻疽患者临床表现及转归的关系。材料与方法采用VITEK 2系统对2018 - 2021年诊断的类鼻疽病患者分离株进行鉴定,并对III型分泌系统基因簇进行聚合酶链反应(PCR)鉴定。采用多重PCR检测脂多糖(LPS) A、B、B2基因型,采用单路PCR检测伯克霍尔德氏菌胞内运动基因(BimA)和丝状血凝素基因(fhaB3)的存在。统计学分析采用卡方/Fisher精确检验研究各种临床表现和转归与不同毒力基因之间的关系。结果用95%置信区间的未调整优势比表示。结果67株菌株可鉴定。BimA - Bm和BimA - Bp分别占82%和18%。脓毒症和死亡率均与BimA - Bm显著相关。大多数分离株含有fhaB3(97%)。多数菌株存在LPS A基因(65.7%),其次为LPS B基因(6%),未检出LPS B2基因。19株分离株不能归属于任何LPS基因型。结论在研究的毒力基因中,只有BimA - Bm与脓毒症和死亡率显著相关。超过四分之一(28.3%)的分离株不能被分配到任何LPS基因型,这表明我们的分离株具有更大的遗传多样性。
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来源期刊
Journal of Laboratory Physicians
Journal of Laboratory Physicians MEDICINE, GENERAL & INTERNAL-
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发文量
99
审稿时长
31 weeks
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