Comparability of 24-hour composite and grab samples for detection of SARS-2-CoV RNA in wastewater.

FEMS microbes Pub Date : 2022-06-17 eCollection Date: 2022-01-01 DOI:10.1093/femsmc/xtac017

Brittany L Kmush, David Monk, Hyatt Green, Darcy A Sachs, Teng Zeng, David A Larsen

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引用次数: 9

Abstract

Wastewater surveillance is a cost-effective way to monitor pathogen prevalence and transmission patterns in the entire community. Here, we compare 24-hour composite and grab samples collected during September 2020 from several municipalities in New York State to detect SARS-CoV-2. A total of 45 paired samples (90 total samples) from three counties and 14 wastewater treatment plants were available for analysis. The categorical comparison (SARS-CoV-2 genetic material detected and quantifiable, genetic material detected but below the limits of quantification, and genetic material not detected) between the grab and composite samples was quite strong, with 91.1% agreement (kappa P-value < .001). The correlations among the quantifiable grab and composite samples were statistically significant yet modest for SARS2-CoV RNA (Pearson correlation = 0.44, P = .02), crAssphage cDNA (Pearson correlation = 0.36, P = .02), and crAssphage DNA (Pearson correlation = 0.46, P = .002). We found good comparison between grab and 24-hour composite samples for detecting SARS-CoV-2 RNA from municipal wastewater treatment plants. Grab sampling is an efficient and cost-effective method to monitor for the presence of SARS-CoV-2 in the entire community.

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废水中SARS-2-CoV RNA检测的24小时复合和抓取样品的可比性

废水监测是监测整个社区病原体流行率和传播模式的一种具有成本效益的方法。在这里，我们比较了2020年9月从纽约州几个城市收集的24小时合成和采集样本，以检测严重急性呼吸系统综合征冠状病毒2型。共有来自三个县和14个污水处理厂的45个配对样本（共90个样本）可用于分析。抓取样本和复合样本之间的分类比较（检测到并可量化的严重急性呼吸系统综合征冠状病毒2型遗传物质，检测到但低于量化限度的遗传物质，以及未检测到的遗传物质）非常强，91.1%的一致性（kappa P值<.001）。可量化的抓取和复合样品之间的相关性在统计学上是显著的，但在严重急性呼吸系统综合征冠状病毒RNA（Pearson相关性=0.44，P=0.02）、crAssphage cDNA（Pearsons相关性=0.36，P=0.02，和crAssphage DNA（Pearson相关性=0.46，P=0.002）。我们发现，在检测城市污水处理厂的严重急性呼吸系统综合征冠状病毒2型RNA方面，抓取样品和24小时复合样品之间有很好的比较。抓斗采样是监测整个社区中严重急性呼吸系统综合征冠状病毒2型存在的一种有效且具有成本效益的方法。

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